Our team performs systematic isolation of B. longum strains from complex microbial sources, followed by selective cultivation and in-depth phenotypic screening. We characterize carbohydrate utilization, SCFA production, and resilience profiles, ensuring candidate strains are accurately identified and validated. By applying genomic and metabolic benchmarks, we help researchers identify promising strains with superior performance for downstream microbiome studies and potential product development.
Creative Biolabs provides targeted functional and mechanism-of-action assessments to uncover the biological roles of B. longum. We evaluate adhesion factors, pili expression, exopolysaccharide production, and metabolic activities such as acetate generation. These assays connect genetic features with phenotypic performance, enabling clients to link specific mechanisms to desired research outcomes. Our detailed datasets empower rational strain selection for advanced applications.
We design in vitro models that mimic gut conditions to investigate how B. longum interacts with host tissues. Using epithelial cell lines, mucus-enhanced environments, and immune co-cultures, we measure adhesion capacity, barrier integrity, and signaling responses. These tests generate strain-specific interaction fingerprints, highlighting unique features such as pili-mediated adhesion or EPS-mediated modulation. Results provide actionable insights into host compatibility and functional potential.
To evaluate immune communication, we perform cell-based assays using PBMCs, macrophages, and reporter systems. Our tests capture cytokine modulation, macrophage polarization, and innate signaling triggered by B. longum or its metabolites. These immune readouts reveal strain-specific patterns that can be directly compared across candidates. Such insights help determine which strains are most suited for immunology-focused microbiome projects and downstream translational exploration.
Formulation is critical for viability and reproducibility. Creative Biolabs optimizes protective matrices such as lyophilization buffers, encapsulation polymers, and cryo-stabilizers. Our team systematically evaluates excipients to improve survival through storage and GI simulation. By linking microbial physiology to formulation outcomes, we provide evidence-driven strategies that ensure B. longum strains remain stable and functional across laboratory and development pipelines.
We create tailored delivery systems to support the efficient application of B. longum. Options include encapsulated powders, freeze-dried sachets, and advanced release vehicles designed for gut-specific delivery. Each design balances viability, release kinetics, and scalability. Our development pipeline ensures strains remain active through handling and use, providing researchers with robust delivery solutions that align with study objectives and experimental needs.
Creative Biolabs supports the rational design of engineered B. longum strains to enhance functional performance in microbiome research. Our team applies adaptive evolution, metabolic pathway tuning, and genetic stability assessments to refine carbohydrate utilization, metabolite output, and adhesion properties. Each engineered strain is carefully validated, ensuring reproducibility and reliability for projects that demand advanced, high-quality microbial design.
Quantify viability decay and functional retention under ICH-like and use-relevant conditions: temperature, humidity, oxygen, and mechanical stress. We incorporate GI simulation (acid/bile) and dynamic resuspension to predict real-world exposure. Encapsulation and protectants are assessed head-to-head to empirically extend shelf life.
