Functional Screening and Mechanism of Action for Live Biotherapeutic Products

The emergence of Live Biotherapeutic allows microbes and probiotics to perform novel therapeutic functions. Creative Biolabs is committed to developing Live Biotherapeutic Products (LBPs), we are flexible to provide customized solutions for your project.

Functions of LBPs

LBPs are thought to exert their effect by modulating the host microbiota. They can inhibit pathogens, produce active molecules/metabolites, modulate the mucosal immune system activity, activate cellular pathways within the epithelial cells, or modulate the activity of the nervous system. Importantly, LBPs will also exert their biological effect by influencing the local ecosystem, influencing other microorganisms and their interactions with the host. Moreover, all or some of the above effects may occur simultaneously, which in turn, will mediate different types of signals, activating diverse physiological pathways within the host.

A schematic of the various target organs and diseases of engineered microbial therapeutics. Fig.1 A schematic of the various target organs and diseases of engineered microbial therapeutics. (Chua, 2017)

Mechanism of LBPs (NGPs) Action

The molecular mechanisms of interaction between the gut commensals and the host cells, and how commensals maintain optimal intestinal integrity and immunity are intensively studied. Probiotics and NGPs influence eukaryotic cells by different mechanisms.

  • Production of Short Chain Fatty Acids (SCFAs)
  • SCFAs are an important source of energy for enterocytes and are key signaling molecules for the maintenance of gut health. In addition, SCFAs can enter the systemic circulation and interact with cell receptors in peripheral tissues. They play an important role in the regulation of energy homeostasis and metabolism. SCFAs can also modulate gene transcription through the inhibition of histone deacetylase activity.

  • Modulation of the Immune System
  • The gut microbiota modulates the immune system via the production of molecules with immunomodulatory and anti-inflammatory functions that are capable of stimulating immune cells. These immunomodulatory effects are due to the interaction of probiotic bacteria with epithelial cells and DCs and with monocytes/macrophages and lymphocytes.

  • Maintenance of Barrier Function in Intestinal
  • Probiotics have been shown to protect the developing epithelia through control of inflammation, upregulation of cytoprotective genes, prevention and regulation of apoptosis, and maintenance of barrier function.

    Therefore, such interactions result in a variety of effects on the host ranging from the improvement of behavior in psychopathological conditions but also impacts on skin health and host metabolism by the mean of immune interaction and Gut-Brain-Skin axis.

How probiotics influence the microbiota and the course of diseases.Fig.2 How probiotics influence the microbiota and the course of diseases. (Wieërs, 2019)

With the advancements in LBP development, a new generation of therapeutics is greatly anticipated in the near future. Creative Biolabs can leverage our rich experience and advanced technology to fast track your LBP development project, offering a new and promising star strain for various diseases. If you are interested in our Functional Screening and Mechanism of Action services in LBP development, please feel free to contact us for more information.

References

  1. Chua, K.J.; et al. Designer probiotics for the prevention and treatment of human diseases. Curr Opin Chem Biol. 2017, 40: 8-16.
  2. Wieërs, G.; et al. How Probiotics Affect the Microbiota. Front Cell Infect Microbiol. 2019, 9: 454.

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