Germ-Free Mouse Model for Studying Bacterial Colonization

Germ-free and gnotobiotic mice are valuable experimental tools for examining host-microbe interactions since mono-colonization of single bacteria is achievable. Creative Biolabs' state-of-the-art next-generation probiotics discovery service platform has significantly accelerated live biotherapeutics research. For each stage of your project, we offer flexible project design and planning, carefully tailoring experimental and technical approaches to best suit your needs.


Germ-free animals constitute a special animal model that is delivered and cultivated in a sterile isolator. Because of their unique aseptic properties, it can be said that germ-free animals offer the possibility to discover the relationship between the host and its microbiota and can be used to study the influence of selected bacterial species on the development of host physiology and pathology. Monocolonization with a restricted bacterial species or mixed microflora (conventionalization) can affect the maturation of the adaptive immune system, the integrity of the mucosal barrier, and the behavioral phenotype. Gut microbiota plays an important role in a variety of physiological functions in the host. The phylogenetic composition of the human gut microbiota can be reconstructed by inoculating the human gut microbiota into a germ-free mouse, humanized gnotobiotic model. These models provide powerful tools for understanding the causality of gut microbiota in humanoid systems. Germ-free animals constitute an ideal model for investigating strain-specific properties by precluding commensal microbiota disturbances.

Probiotic Colonization of the Intestinal Mucosa

Successful colonization of the gastrointestinal tract is a key factor for probiotics to be able to exert sufficient host interaction to confer a health benefit. Mucosal adhesion is considered a critical step in probiotic colonization. Mucosal adhesion is considered a key step in probiotic colonization. On the premise of overcoming colonization resistance, the colonization of probiotics in the mucus layer can be realized through the binding of bilateral adhesive proteins. How probiotics communicate with commensal bacteria and some are successfully introduced to gut microbiota is also of great interest. Understanding these factors will help design effective delivery strategies using probiotics to overcome colonization resistance and achieve health benefits.

Various factors affect the viability of probiotics during gastrointestinal transit.Fig.1 Various factors affect the viability of probiotics during gastrointestinal transit. (Han, 2021)

Bacterial Colonization Services at Creative Biolabs

  • Bacterial Strain Culturing
  • Strain Transplantation via Oral Gavage
  • Tissue Collection
  • Histopathological Analysis
  • Phenotypic Assessment

Products for Bacterial Colonization Research at Creative Biolabs

  • Probiotic strain products
  • Customized Strain Culture Supernatant.
  • Customized strain lyophilized powder containing certain CFU.

Creative Biolabs' scientific expertise, advanced in-house technology, and top-notch germ-free mice models mean our team can provide high-quality support for your project. Contact us to establish a free consultation and learn more about our full range of services.


  1. Han, S.; et al. Probiotic gastrointestinal transit and colonization after oral administration: A long journey. Frontiers in Cellular and Infection Microbiology. 2021, 11: 609722.

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