Engineering Lactobacillus bulgaricus Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) is widely used in the food industry, and this generally recognized as safe (GRAS) bacteria is used worldwide in yogurt production in cooperation with Streptococcus thermophilus. Despite the significant commercial value of Lactobacillus bulgaricus, the lack of effective genetic engineering tools has seriously hindered the better utilization of this microbe's potential as a GRAS host for the production and secretion of foreign proteins. Since the production of biologically significant output proteins depends on the efficiency of expression and secretion, the validation of basic tools such as the strong promoter of L. bulgaricus and the reporting of secreted proteins still need to be developed. L. bulgaricus is considered to be a probiotic that has been labeled as a friendly bacteria and is found in the enteric system as well as in the oral cavity. Intestinal bacterial therapy is a new therapy for chronic renal failure. It is amenable to oral administration as a micro-capsule containing the entire organism directly yielding the enzyme or expressing exogenous enzyme through genetic engineering. In recent years, engineered bacteria have been used to clear urine toxins and used as biological agents. Currently, engineered bacteria only produce a single enzyme, such as urease and uricase.
L. bulgaricus Genetic Engineering Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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PAGE
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or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain
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Target gene name
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Target gene sequence, if the whole genome sequence is unavailable
How L. bulgaricus Strain Construction Service Can Assist Your Project
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Construct the target strains according to the needs of clients or refer to the published scheme.
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Optimize the program to improve the success rate.
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Delivery of the constructed target strains (knockout or integrated).
Advantages
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Due to the wide application of L. bulgaricus, it is urgent to conduct in-depth genetic research on L. bulgaricus to maximize its utilization value.
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The whole genome sequence data of 4 strains of L. bulgaricus were obtained in the gene and genome database.
Frequently Asked Questions
How does L. bulgaricus affect humans?
When it is consumed, it is called a probiotic. A proper balance of gut flora helps keep your gut walls strong and harbor bad bacteria, which can reduce the risk of chronic disease. Probiotics are "good" bacteria that play an important role in maintaining health.
What is L. bulgaricus used for in food?
Yogurt is a product made from L. bulgaricusand Streptococcus thermophilus that curdles milk under the action of lactic acid. Milk-derived proteins are good immune stimulants and are therefore used in therapy.
Published Data
Lactobacillus bulgaricus inhibits colitis-associated cancer via negative regulation of intestinal inflammation in azoxymethane/dextran sodium sulfate model.
Abstract
To investigate the role of L. bulgaricus in the colitis-associated colon cancer (CAC) model. The probiotic group was given L. bulgaricus daily. Intestinal inflammation was determined by clinical symptom score. It was found that L. bulgaricus had an inhibitory effect on tumor volume and mean tumor size. In addition, probiotics can also reduce the clinical symptoms of intestinal inflammation. The research results indicate that L. bulgaricus has anti-inflammatory and anti-tumor effects in colitis-related carcinogenesis, and may play an important role in the prevention and treatment of CAC in the future.
Fig.1 Lactobacillus bulgaricus inhibits tumor progression in azoxymethene/dextran sulfate sodium-exposed mice.1
Resources
Creative Biolabs has accumulated extensive experience in the development of live biotherapeutics and is familiar with a wide range of probiotics. If you are interested in our Lactobacillus bulgaricus engineered strains construction services, please do not hesitate to contact us for more details, we look forward to helping you at some stage of the project.
Reference
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Silveira, Denise Sayuri Calheiros, et al. "Lactobacillus bulgaricus inhibits colitis-associated cancer via a negative regulation of intestinal inflammation in azoxymethane/dextran sodium sulfate model." World Journal of Gastroenterology 26.43 (2020): 6782.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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