Engineering Lactobacillus rhamnosus Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
Among Lactobacillus strains, the most widely studied is Lacticaseibacillus rhamnosus, whose predecessor was Lactobacillus rhamnosus. The GG strain of Lactobacillus rhamnosus is the most well-documented probiotic and has been identified as a potential probiotic strain. Probiotic engineering is a promising strategy for developing probiotic strains. However, although many of the probiotic properties of Lactobacillus rhamnosus have been proven to be beneficial, there is still limited research on its bioengineering applications. In some studies, recombinant Lactobacillus rhamnosus can effectively reduce the incidence, severity, and duration of diarrhea, while other reported strains of recombinant Lactobacillus rhamnosus can be used to inhibit HIV transmission and replication, ultimately inhibiting disease progression. Due to the ability of recombinant Lactobacillus rhamnosus to host the immune system, more and more researchers are exploring its use as a feasible delivery carrier to deliver preventive and therapeutic molecules to the mucosal surface. These studies, although not numerous, suggest that different researchers are studying recombinant Lactobacillus rhamnosus for different purposes.
Engineering Lactobacillus rhamnosus Services
Services
-
Gene knock-out
-
Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
-
Sequencing Validation
-
Western Blot Validation (Optional)
-
PAGE
-
or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
-
Overall construction scheme.
-
Host strain
-
Target gene name
-
Target gene sequence, if the whole genome sequence is unavailable
How Lactobacillus rhamnosus Strain Construction Service Can Assist Your Project
-
Construct the target strains according to the needs of clients or referring to the published scheme.
-
Optimize the program to improve the success rate.
-
Delivery of the constructed target strains (knockout or integrated).
Advantages
-
The use of L. rhamnosus engineering strategies may result in more beneficial effects on the host.
-
Generation of newly engineered L. rhamnosus strains by cloning and expressing different genes into probiotics will confer beneficial effects of the probiotic, as well as traits resulting from the expression of additional foreign genes or genes.
-
The development of the gene editing system in combination with ssDNA recombineering would allow the editing of the genome in probiotic bacteria to acquire some new properties without antibiotic resistance.
Frequently Asked Questions
Why is Lactobacillus rhamnosus a suitable candidate for constructing novel bioengineered probiotic strains?
The development of recombinant probiotic strains with dual action potential is an interesting avenue. The ability of L. rhamnosus to enhance beneficial effects compared to other probiotics makes it a suitable candidate for the development of recombinant strains with a high probability of successful development of novel robust probiotic strains with enhanced health benefits.
What are the research applications of bioengineered Lactobacillus rhamnosus?
Bioengineered strains of Lactobacillus rhamnosus can be designed for target control of many pathogens. Currently, the use of probiotics as a delivery vehicle is gaining momentum, so leveraging beneficial bacteria such as Lactobacillus rhamnosus is a good strategy with a good chance of success in developing new robust probiotic strains with enhanced health benefits.
Published Data
Lactobacillus rhamnosus probiotic prevents airway function deterioration and promotes gut microbiome resilience in a murine asthma model
Abstract
To study the preventive and therapeutic effects of wild-type and recombinant Lactobacillus rhamnosus GR-1 on experimental allergic asthma in mice. Administration of wild-type and recombinant Lactobacillus rhamnosus GR-1 can prevent airway hyperreactivity. Recombinant Lactobacillus rhamnosus GR-1 also prevented total airway cell count, lymphocyte count, and lung IL-1 β an increase in level. These data suggest that Bet v1 expression in L. rhamnosus GR-1 further promotes airway inflammation.
Fig.1 Production of Bet v 1 by the L. rhamnosus GR-1-derived strain using the NICE expression system.1
Resources
Creative Biolabs has accumulated rich experience in the field of probiotics research. We have established advanced gene editing technology platforms and launched microbial genome editing services for gene knock-in, knockout, or gene replacement in Lactobacillus rhamnosus. If you are engaged in this research, you are welcome to contact us at any time to exchange and discuss our services or technology.
Reference
-
Spacova, Irina, et al. "Lactobacillus rhamnosus probiotic prevents airway function deterioration and promotes gut microbiome resilience in a murine asthma model." Gut Microbes 11.6 (2020): 1729-1744.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Related Services
