Comprehensive & Reliable Probiotic Anti-Cancer Efficacy Assay

In Vitro Assays (Cell-Based Studies)

• Cytotoxicity Assays
  
  • Investigate the direct cytotoxic impact of probiotics or their metabolic byproducts on diverse cancer cell lines.
  • Comparing cytotoxicity against normal cell lines to assess selective toxicity.
• Anti-proliferation Assays
  
  • Measuring the ability of probiotics to inhibit the growth of cancer cells.
  • Analyzing cell cycle progression to identify potential arrest points induced by probiotics.
• Apoptosis and Necrosis Assays
  
  • Determining if the anti-cancer effects involve programmed cell death or uncontrolled cell death.
• Invasion and Metastasis Assays
  
  • Evaluating the impact of probiotics on the ability of cancer cells to invade through extracellular matrix and migrate.
• Modulation of Cancer-Related Signaling Pathways
  
  • Investigating the effects of probiotics on key signaling pathways involved in cancer development and progression.
• Angiogenesis Assays
  
  • Assessing the potential of probiotics to inhibit the formation of new blood vessels that support tumor growth using in vitro angiogenesis assays.
• Immunomodulation Assays
  
  • Evaluating the impact of probiotics on immune cells and the production of cytokines and chemokines in the context of cancer.
• Analysis of Probiotic Metabolites
  
  • Identifying and quantifying metabolites produced by probiotics and assessing their direct anti-cancer effects.
• Gene Expression Analysis
  
  • Examining the changes in gene expression in cancer cells treated with probiotics to understand the underlying mechanisms of action.
• 3D Cell Culture Models (Spheroids, Organoids)
  
  • Utilizing more complex in vitro models that better mimic the tumor microenvironment to assess probiotic efficacy.

In Vivo Assays (Animal Studies)

• Efficacy Studies
  
  • Evaluating the ability of probiotics to inhibit tumor growth, reduce tumor burden, and prolong survival in preclinical animal models.
  • Administering probiotics through different routes.
• Metastasis Studies
  
  • Assessing the impact of probiotics on the development and progression of metastasis in vivo.
• Pharmacokinetic and Pharmacodynamic (PK/PD) Studies
  
  • Investigating the distribution, metabolism, and excretion of probiotics or their bioactive compounds in vivo, as well as their effects on tumor markers and the host immune system.
• Immunohistochemistry and Histopathology
  
  • Assess the effects of probiotics on tumor cell proliferation, apoptosis, angiogenesis, and immune cell infiltration.
• Analysis of Gut Microbiota Composition
  
  • Evaluating the changes in the gut microbiome of tumor-bearing animals treated with probiotics using techniques like 16S rRNA sequencing or metagenomics.
• Combination Therapy Studies
  
  • Assessing the synergistic or additive effects of probiotics in combination with conventional cancer therapies.

Additional Services

• Custom Assay Development: Tailoring assays to meet specific research questions and probiotic candidates.
• Strain Characterization: In-depth analysis of the probiotic strains being tested (e.g., identification, genomic analysis).

Deliverables

The general final deliverables of services for probiotic anti-cancer assays are comprehensive packages of information and data that address the research questions and provide a clear understanding of the probiotic's potential.

Turnaround Time

The typical timeframe for this service ranges from 4 to 8 weeks, depending on the complexity of the experimental design, the number of probiotic strains and conditions to be tested, and the specific assays required.