In Vivo Tools for Live Biotherapeutic Products Assessment

Animal models have been used to solve a variety of scientific problems, from basic science to the development and evaluation of new vaccines or therapeutics. In the rapidly growing field of live biotherapeutics, Creative Biolabs' in vitro tools enabled us to help our clients research, discover, and develop new live biotherapeutics and next-generation probiotics.

Animal Models in Translational Medicine

A disease model should mimic the clinical disease condition as much as possible allow the investigation of unclear pathophysiological features towards the confirmation/negation of a mechanistic relationship and assure a reliable development of new potential therapeutic options. Animal models provide an invaluable means to study complex physiological and biochemical interactions that cannot be fully simulated in silico and/or in vitro.

In Vivo Assessment of Safety

Animal models are commonly used to mimic the pathogenesis of infectious or inflammatory diseases. They should reproduce at least some of the risk factors that favor the expression of potential virulence factors in lactic acid bacteria. Based on the expertise, the rat endocarditis and the immunocompromised mouse model were chosen for in vivo assessment of safety.

  • Experimental Endocarditis

The rat model of experimental endocarditis mimics the physiology and pathology of human infective endocarditis. The rat endocarditis model can be used to test the potential virulence characteristics of novel probiotics for safety assessment.

  • Immunocompromised Mouse Model

Immunodeficient mouse models were designed to mimic immunodeficient patients. The method works well for detecting intestinal probiotics and can detect differences between strains carrying virulence genes and those deprived of virulence genes.

In Vivo Tools for Live Biotherapeutics Discovery at Creative Biolabs

When specifically considering the interactions of probiotics with host microbiota, a deep understanding of the symbiosis between animals and bacteria is key to understanding the complex biology of environmental, genetic, and microbiome interactions relevant to the evolution of human health and disease. While taking into account the dynamics of the human microbiome across life stages, the predictive value of simple models may indeed have its limitations, their use may be crucial for understanding the mechanisms of interaction and the regulation of metabolic, physiological, and immune activities.

When studying in vivo models of LBP risk recording, simple animal models such as Drosophila melanogaster or Caenorhabditis elegans may help decipher pathways within the host. Immunodeficient D. melanogaster could offer a platform to screen microbe-xenobiotic in vivo interactions. C. elegans could be a suitable model to understand whether the host, the microbe, or the environment, determines the susceptibility or resistance to infections.

Several mammalian models have been used to study acute and chronic intestinal inflammation. Studies of germ-free mice have helped determine the role of gut microbiota in shaping the gut immune system. Small rodents, including rats and especially mice, are the most widely used preclinical animal models for studying metabolic disorders.

Progressive complexity of in vivo models used to support probiotic health effects.Fig.1 Progressive complexity of in vivo models used to support probiotic health effects. (Papadimitriou, 2015)

Creative Biolabs is an outstanding company that provides quality products and best-in-class customer service to the world's leading pharmaceutical, biotechnology, contract research organizations, and universities. If you are interested in our in vivo tools for live biotherapeutics research, or if you can not find an animal model of interest on our website, please do not hesitate to contact us for assistance.

Reference

  1. Papadimitriou, K.; et al. Discovering probiotic microorganisms: in vitro, in vivo, genetic and omics approaches. Frontiers in microbiology. 2015, 6: 58.

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