As research scientists dedicated to advancing therapeutic strategies against fungal pathogens, we at Creative Biolabs offer a suite of meticulously validated antifungal testing solutions. Driven by our scientific rigor and a shared goal of combating fungal diseases, Creative Biolabs delivers high-precision, reliable antifungal testing with efficient timelines. This directly fuels pivotal stages of your antifungal research and development pipelines. Acknowledging the multifaceted challenges of drug discovery and preclinical studies, we offer a comprehensive portfolio of methodologies and the adaptability necessary to craft solutions precisely suited to your distinct experimental demands. Request a quote
Incubation
Cultivate the test substances under appropriate conditions to ensure that the fungi grow without antifungal agents.
Reading and Interpretation of Results
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After incubation, evaluate the growth of the fungi in the presence of different concentrations of antifungal agents.
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MIC: The minimum concentration of the antifungal agent that inhibits the visible growth of fungi is judged as MIC.
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Determine the minimum effective concentration (MEC): The lowest concentration at which mycelial growth is significantly hindered compared with the control.
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Result interpretation.
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Quality Control: Set up controls throughout the entire testing process to ensure the sterility of the culture medium and reagents, that is, the applicability of the operating process.
Deliverables
The definitive outputs of our antifungal activity testing furnish a thorough evaluation of a fungal isolate's responsiveness to a panel of antifungal agents. These results are delivered in a standardized, readily interpretable format, accompanied by detailed methodological descriptions and rigorous quality control metrics. This information is paramount for informing effective antifungal treatment strategies and for advancing fundamental research in the field.
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Deliverables
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Details
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Formal Report
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Sample Information
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Antifungal Agent Panel
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Testing Methodology
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Results of Antifungal Susceptibility Testing
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MIC (Minimum Inhibitory Concentration): Expressed in μg/mL or mg/L.
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Zone Diameter (if Disk Diffusion was used): Measured in millimeters.
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MEC (Minimum Effective Concentration) (if applicable): Representing significant morphological growth inhibition.
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Interpretation
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Turnaround Time
The typical timeframe for this service ranges from 3 to 6 weeks, depending on the complexity of the project, the number of isolates and antifungal agents, and the specific assays required. Project timelines will be clearly defined in the initial consultation and project proposal.
Case 1: Dose-response Assays of the Selected Molecules Against Candida albicans Biofilms (XTT Assay)
Sample: Compound was Provided by the client.
Fungus: (Provided by Creative Biolabs.)
The client provided two small molecule samples. We contracted for the dose-response assay to test the inhibitory effects on the Candida albicans biofilm formation. As shown in the figure, the molecule could inhibit biofilm formation.
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Comprehensive Methodologies
We provide a wide range of AFST methods, including broth microdilution (CLSI and EUCAST), gradient diffusion, disk diffusion, automated testing, and molecular methods, ensuring we can meet diverse research needs.
Accuracy and Reliability
We adhere to strict quality control procedures and follow CLSI and EUCAST guidelines, ensuring the accuracy and reliability of our results.
Customized Solutions
We offer customized AFST panels and study designs tailored to your specific research objectives and fungal species.
Rapid Turnaround Times
Our streamlined workflows and state-of-the-art equipment enable us to deliver results on time, accelerating your research progress.
Expert Support
Our team of experienced microbiologists and infectious disease specialists provides expert consultation, data analysis, and interpretation.
State-of-the-Art Technology
Creative Biolabs utilizes advanced automated systems like VITEK 2, for high-throughput and rapid AFST.
Molecular Resistance Detection
In addition to phenotypic methods, Creative Biolabs offers molecular methods, such as PCR-based assays, to detect specific genetic mutations associated with antifungal resistance.
Quality Assurance
Creative Biolabs maintains rigorous quality control procedures, adhering to CLSI and EUCAST guidelines to ensure the accuracy and reliability of AFST results.
Can Creative Biolabs help with non-routine antifungal susceptibility testing?
Yes, we focus on developing and validating customized analyses to address unique research challenges.
What quality control measures are in place to ensure the accuracy of the results?
Creative Biolabs adheres to strict quality control procedures, following CLSI and EUCAST guidelines. We use calibrated equipment, validated methods, and reference strains to ensure the accuracy and reproducibility of our results. We also provide detailed quality control reports with every project. Inquire today for more details on our quality assurance process.
What types of fungal species and antifungal agents can Creative Biolabs test?
Creative Biolabs can test a wide range of fungal species, including yeasts and molds relevant to research.
