From fecal samples or strain libraries, Creative Biolabs selectively enriches and purifies A. hallii under strictly anoxic conditions. We establish a high-throughput screen centered on butyrate yield and lactate clearance, integrating automated colorimetric/GC readouts and robotics to rapidly prioritize A. hallii isolates exhibiting robust growth, stable colony morphology, and reproducible metabolic performance across representative substrates.
Using 16S rRNA sequencing, whole-genome sequencing, and metabolic fingerprinting, Creative Biolabs verifies A. hallii identity and purity. Key butyrate-pathway loci are annotated, while plasmids and SNPs are tracked for genetic stability. Comparative genomics supports seed-lot qualification, ensuring authenticated A. hallii working stocks aligned with protocol design, data integrity, and chain-of-custody, over time and across passages.
We quantify A. hallii utilization of lactate, acetate, monosaccharides, and selected resistant-carbohydrate hydrolysates, resolving SCFA output by GC. We map growth kinetics, yields, and co-substrate preferences, capturing lactate-to-butyrate stoichiometry, acetate balances, and residuals. Results guide media formulation and co-culture strategies that favor A. hallii cross-feeding, stability, and highly reproducible metabolite envelopes in complex communities.
We develos and scale strictly anaerobic fermentations for A. hallii with controlled pH, redox, and dissolved gases. Bioreactor runs tune agitation, sparging, and hydrogen partial pressure to sustain redox balance and butyrate productivity. In-process analytics track OD, metabolites, and viability, enabling translation from serum vials to stirred tanks and pilot-ready configurations at multiple scales.
Because A. hallii is oxygen-sensitive and stress-responsive, Creative Biolabs screens cryo- and lyoprotectants, bulking agents, and antioxidants. We design freeze-drying or frozen logistics with oxygen-scavenging packaging and humidity control, optimizing residual moisture, survival, and phenotype retention. Stability studies bracket storage temperatures and transit durations, preserving A. hallii viability and functional outputs throughout distribution and handling.
Creative Biolabs engineers A. hallii formulations using microencapsulation, polymeric matrices, and enteric carriers compatible with tailored prebiotics. Release testing evaluates acid resistance, bile-tolerance surrogates, and timed de-encapsulation. We tune particle size, surface charge, and excipient ratios to support A. hallii delivery, persistence in simulated gut environments, and rational pairing with partner strains for efficient cross-feeding.
Creative Biolabs quantifies A. hallii functional outputs including lactate clearance, butyrate formation rates, NAD(H) coupling, and electron-transfer efficiency. High-content assays capture cofactors, metabolite fluxes, and interactions with complementary commensals, mapping cross-feeding networks. Findings prioritize A. hallii strains with consistent mechanisms and performance envelopes suitable for hypothesis-driven microbiome research programs and robust decision-making across relevant conditions.
In epithelial and mucus analogs, organoids, and barrier-on-chip systems, Creative Biolabs profiles A. hallii effects on TEER, tight-junction proteins, and SCFA-responsive signaling readouts. We apply conditioned media, cell-free supernatants, or defined co-culture to parse contact-dependent versus metabolite-mediated responses, translating A. hallii function into quantifiable, reproducible endpoints for discovery-stage research programs with standardized controls and benchmarking.
