Germ-free Mouse Model for Studying Host-microbial Interactions

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It is difficult to characterize the effects of probiotics on host function in vivo studies of conventionally reared mice, but probiotics can alter host physiological functions, as well as affect the host immune system, and have secondary effects on host microbial communities and their functions. To better understand how individual microbial species, either resident members of the gut or probiotic species, affect host physiology we need a simplified model system that can help control the off-target effects of these bacteria. Gnotobiotic mice represent such a system in which we can introduce microorganisms singly or serially and study the effects of individual bacteria or known microbiota on host function.

Germ-free Mice Model as a Tool for Studying Host-microbial Interactions

Experimental models utilizing germ-free animals are invaluable tools for understanding how the gut microbiota may affect host physiology and metabolism, or for exploring interactions between the host and its microbiota. Inoculation of human gut microbiota into germ-free mice, humanized gnotobiotic models, allows recapitalization of the human microbiota phylogenetic composition. These models provide powerful tools for understanding the causality of gut microbiota in humanoid systems. Gnotobiotic mice allow the study of microbe-microbe interaction to better understand how the introduction of a new microbial member affects community membership and function. Germ-free animals are valid experimental models used to investigate the host-microbial interactions in health, disorders, and diseases. Germ-free animal models are valuable experimental tools for host-microbe interaction research in many fields such as cancer therapy, metabolism, diabetes, reproduction, cardiovascular problems, and bone homeostasis.

  • Understand the role of the gut microbiome in obesity.
  • Understand the effect of microbiota on the development and function of the immune system.
  • Understand the effects of microbes on GI physiology including motility and secretion.

Germ-free mice as a tool to study the microbiota-gut-brain axis.Fig.1 Germ-free mice as a tool to study the microbiota-gut-brain axis. (Luczynski, 2016)

In Vivo Testing Services at Creative Biolabs

Germ-free animal model is a very useful tool to study microbe-microbe and microbe-host interaction in health and disease as well as a preclinical model to test microbiota-directed therapies.

  • Disease treatment efficacy of probiotics
  • Strain transplantation via oral gavage
  • Single probiotic strain culturing
  • Phenotypic assessment
  • Tissue collection

Creative Biolabs' Products for Germ-free Mice Model Research

  • Probiotic strain products
  • Customized strain culture supernatant.
  • Customized strain lyophilized powder containing certain CFU.

Creative Biolabs has always been committed to providing high-quality research products and services to our customers. Our unparalleled platform and outstanding next-generation probiotics discovery expertise enable us to provide you with comprehensive data and unique insights, giving you the depth and breadth of science to advance your drug discovery program. Please feel free to contact us to discuss your Germ-free mice model research with gut microbiota.


  1. Luczynski, P.; et al. Growing up in a bubble: using germ-free animals to assess the influence of the gut microbiota on brain and behavior. International Journal of Neuropsychopharmacology. 2016, 19 (8).

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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