Antibiotic Susceptibility/Resistance Test for Live Biotherapeutic Products

Creative Biolabs has been focusing on the development of high-quality live biotherapeutics products for the treatment of a diversified battery of diseases, including autoimmune diseases, cancers as well as infectious diseases. Equipped with a team of professional scientists, we are capable of providing our clients with a variety of customized services in antibiotic susceptibility/resistance tests. We guarantee the finest results for our customers all over the world.

Introduction to Antibiotic Susceptibility/Resistance

In recent years, live bacterial therapeutics has become a new class of regulatory approach for treating various human and animal diseases. For instance, the development of novel next-generation probiotics (NGPs) can be widely used for skin disease control with a high therapeutic effect and improved safety. However, pilot studies have indicated that most NGPs strains are susceptible to certain types of antibiotics, such as ampicillin and gentamicin, and they are not be easily eliminated if they harm the host. Moreover, the antibiotic resistance of these NGPs may subsequently spread to specific pathogenic microorganisms. As a result, a panel of assays, including antibiotic susceptibility/resistance testing, have been generated for measuring the pathogenic bacteria in clinical samples.

Fig.1 Representation of various conventional antibiotic susceptibility testing methods. (Khan, 2019)

Our Services for Antibiotic Susceptibility/Resistance Test

Antibiotic susceptibility/resistance has been regarded as a growing global health concern. The potentially pathogenic bacteria of live biotherapeutic products have developed resistance to particular or multiple antibiotics. Infections with antibiotic-resistance bacteria have become the main cause of tumors and heart diseases. Therefore, several susceptibility testing assays have been performed on different live biotherapeutic product samples to detect the ability of antibiotics to inhibit the growth of NGPs candidates.

As the long-term pioneer as well as the market leader in the field of live biotherapeutics drug discovery, Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in antibiotic susceptibility/resistance testing with our customers.

In our labs, the samples and antibiotics can be cultured in nutrient media to directly measure the growth of the bacteria. Furthermore, the resistance to specific antibiotics can also be assessed by detecting the expression level of genes that are associated with antibiotic resistance. In a recent study, the antibiotic susceptibility/resistance property of a live biotherapeutic product,asymptomatic bacteriuria Escherichia coli 2-12, has been evaluated in dogs with urinary tract infection. The data have suggested that this strain is resistant to five antibiotics, such as lincomycin. Antibiotic susceptibility/resistance plays an important role in screening suitable NGPs in live biotherapeutics drug discovery.

Methods of Antibiotic Susceptibility/Resistance Test

With years of operational experience, Creative Biolabs can provide state-of-art services for any live biotherapeutics drug discovery project. Nowadays, we have developed efficient protocols for antibiotic susceptibility/resistance testing. We can also conduct custom in vitro safety studies to meet any requirements of our clients. Taking advantage of rapid diagnostic methodologies, we have developed, optimized, and validated a panel of assays, which can generally be sorted into six distinct types, including but not limited:

• Phenotypic-based testing assays
• Disk diffusion assays
• Epsilometer testing (Etest) assays
• MALDI-TOF MS assays
• PCR-based testing assays
• Microfluidics-based assays

As a well-recognized expert in the field of live biotherapeutic products evaluation, Creative Biolabs has achieved a lot of projects for our clients based on our extensive experience and advanced platform. We are therefore confident in offering the best antibiotic susceptibility/resistance testing services to customers all over the world. Besides, our scientists specialized in new NGPs discovery will work with you to develop the most appropriate strategy that will offer the most meaningful data for your research. Contact us to discuss your project and experience the great value of our services.

Reference

1. Khan, Z. A., et al. Current and emerging methods of antibiotic susceptibility testing. Diagnostics. 2019, 9(2): 49.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.