Antagonistic Activity Assay for Next-generation Probiotics
With our innovative technology and team of experts, Creative Biolabs is supporting the scientific community to achieve its goals, increase its understanding of the next generation of probiotics, and make discoveries. We should be the best partner for live biotherapeutics to make breakthroughs possible.
Overview
An important property of probiotics is their antagonistic or antibacterial action against pathogens. This is achieved by competitive exclusion and/or production of bacteriocins or bacteriocin-like substances and organic acids, thereby regulating the gut microbiota. Probiotics are being used widely as one of the key solutions to restore the balance of the gut microbial ecosystem by controlling pathogens and promoting beneficial functions of the gut, thereby improving or preventing intestinal or systemic disease phenotypes by controlling pathogens and promoting beneficial functions of the gut. Lactic acid bacteria have different in vitro antibacterial mechanisms. The antagonistic activity in liquid media is favored by rapidly diffusing anti-microbial compounds, including organic acids and co-aggregation of different indigenous bacteria with pathogens. Antagonistic activity of probiotic strains is mainly attributed to the production of metabolites such as organic acids, hydrogen peroxide, ethanol, acetaldehyde, acetoin, carbon dioxide, reuterin, and other bacteriocins, as well as competitive exclusion, immune regulation, stimulation of host defenses, and production of signaling molecules that trigger changes in gene expression.
Fig.1 Interactions of E. coli strain Nissle 1917 with the intestinal microbiota and with the host. (Sonnenborn, 2016)
The Antagonistic Mechanisms of Probiotics
The mechanisms underlying the antagonistic effects of probiotics on various microorganisms include the following:
-
The secretion of antimicrobial substances.
-
The competitive adherence to the mucosa and epithelium.
-
The strengthening of the gut epithelial barrier.
-
The modulation of the immune system.
Antagonistic Activity Assays at Creative Biolabs
Bacteria can produce a variety of antimicrobial substances. In terms of the maintenance and improvement of intestinal microbial balance, many studies have primarily focused on the antagonistic effects of probiotic isolates against pathogens as important criteria for selecting probiotics. Most studies have been carried out using a single strain of probiotics and a different selection of pathogenic bacteria by in vitro agar spot assay or well diffusion assay.
-
Anti-microbial activity on agar plates
-
Anti-microbial activity in broth
-
Antagonistic activity of the culture supernatants
Creative Biolabs has an experienced research team and a leading platform to provide customers with comprehensive probiotics research services. According to the needs of customers, to achieve precise customized services for customers. If you encounter difficulties in your project research and need help, please do not hesitate to contact us. Our team will communicate with you comprehensively to understand your needs.
Reference
-
Sonnenborn, U. Escherichia coli strain Nissle 1917-from bench to bedside and back: history of a special Escherichia coli strain with probiotic properties. FEMS microbiology letters. 2016, 363(19): fnw212.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Related Services
