Creative Biolabs isolates and screens P. freudenreichii strains from dairy matrices, artisanal cheeses, silage, and microbiome samples using selective, long-incubation anaerobic protocols. We combine colony morphology, lactate-to-propionate conversion, SCFA profiles, and vitamin B-group production data to shortlist strains with strong growth, propionate output, and probiotic-like properties for downstream development.
Our team performs precise identification of P. freudenreichii using 16S rRNA sequencing, whole-genome sequencing, and high-resolution comparative genomics. We assign strains unambiguously, profile plasmids and mobile elements, and generate traceable, regulatory-ready documentation that supports food, feed, and live biotherapeutic research pipelines requiring full genetic and taxonomic transparency.
Creative Biolabs designs and optimizes anaerobic and microaerophilic fermentation processes tailored to P. freudenreichii, controlling pH, redox potential, lactate supply, and gas composition to maximize biomass, propionic and acetic acid, and active vitamin B12 production. We transfer conditions from serum bottles to lab- and pilot-scale bioreactors, always with industrially relevant, feedstock-efficient process parameters in mind.
We develop scalable downstream workflows for P. freudenreichii, including continuous harvesting, gentle cell concentration, washing, and stabilization strategies compatible with frozen, liquid, spray-dried, or freeze-dried formats. Process parameters are tuned to preserve viability, stress-response proteins, and SCFA-related metabolic capacity, ensuring performance continuity from fermentation to finished experimental formulations.
P. freudenreichii is known for its multi-stress tolerance, but responses differ markedly by strain. We profile how P. freudenreichii handles acid, bile, osmotic load, oxygen, temperature shifts, and drying. High-content assays quantify survival, membrane integrity, and key stress-response markers, guiding rational selection of robust strains for gut, oral, feed, or non-dairy food matrices.
To uncover how P. freudenreichii works in real systems, Creative Biolabs conducts functional and MoA screens focused on SCFA production, bifidogenic factor secretion, and vitamin B12 output, as well as immunomodulatory signatures in relevant in vitro models. We link metabolite fingerprints to cytokine patterns and barrier readouts, enabling evidence-based positioning of individual strains or consortia.
Using epithelial monolayers, mucus-producing co-cultures, and immune cell co-culture systems, we assess how P. freudenreichii interacts with host cells. Readouts include barrier integrity, tight-junction marker expression, cytokine modulation, and microbiome-interaction endpoints such as bifidobacteria stimulation in multi-species communities, reflecting published evidence for immunomodulation and gut microbiota reshaping.
Creative Biolabs engineers and optimizes P. freudenreichii strains for enhanced stability, metabolite yield, and target functionality. We apply CRISPR-ready genetic tools where appropriate, together with adaptive evolution and DoE-based process optimization, to fine-tune B-vitamin biosynthesis, propionic acid output, and stress tolerance. These engineered or optimized strains provide a strong foundation for next-generation probiotic and functional food concepts.
