Propionibacterium freudenreichii as Next Generation Probiotics

Propionibacterium freudenreichii (P. freudenreichii), a probiotic candidate, is known for producing propionate as a by-product of lactate fermentation. It is considered a next-generation probiotic (NGP) that can confer health benefits on the host. Creative Biolabs specializes in NGPs development. Our expert scientists and talented technical support team have got you covered all the way for your P. freudenreichii research.

Introduction of P. freudenreichii

P. freudenreichii is a gram-positive bacterium usually used to produce fermented dairy products, especially in ripening culture in the manufacture of Swiss-type cheese. The cells most commonly take the shape of pleomorphic rods, but they can also be coccoid, bifid, branched, or filamentous. When grown on solid media, the colonies formed may appear rough, or smooth, convex. When grown in liquid media the colonies may be observed, appearing granular and varying in size. P. freudenreichii is a probiotic that is generally considered safe. It can be used as a cheese starter and can produce nutrients such as vitamin B12, B2, and K with bifidobacterium activity.

Probiotic supplementation dampens the systemic inflammatory response to fracture. Fig.1 Scanning electron micrograph of a freeze-dried culture (top) and a light micrograph (bottom) of a liquid culture of P. freudenreichii. (Fröhlich, 2017)

P. freudenreichii for Disease Treatment

Scientists investigated the anti-obesity and anti-diabetes effects of P. freudenreichii MJ2 isolated from raw milk using 3T3-L1 cells and obese mice induced by a high-fat diet (HFD). The results suggested that the potential probiotic P. freudenreichii MJ2 inhibits lipid accumulation and reduces obesity and obesity-related metabolic disorders, including hyperlipidemia, type 2 diabetes, and liver damage. Live and heat-killed P. freudenreichii MJ2 had a similar anti-obesity effects, suggesting that some components of P. freudenreichii MJ2 that have not been inactivated by heat may improve obesity. This is an important advantage in developing P. freudenreichii MJ2 as a drug or functional food for the treatment of obesity. Therefore, P. freudenreichii MJ2 is a potential probiotic to improve obesity by regulating lipid metabolism.

Effect of MJ2 and hkMJ2 on lipid accumulation in the livers of HFD-induced obese mice. Fig.2 Effect of MJ2 and hkMJ2 on lipid accumulation in the livers of HFD-induced obese mice. (An, 2021)

  • Acute Colitis

The effects of P. freudenreichii-induced mucin stimulation on colitis rat model and LS 174T goblet cells were observed in vitro and in vivo. The mRNA and protein expression levels of MUC2, the main component of intestinal mucus, increased in the supernatant of P. freudenreichii culture (SPFC)-treated LS 174 cells. After treatment with SPFC or live P. freudenreichii (LPF), the mRNA levels of typical pro-inflammatory cytokines decreased, and the inflammatory state was histologically improved in the rats with DSS-induced colitis. In addition, SPFC and LPF increased the level of propionate in the feces of rats with DSS-induced colitis. Therefore, P. freudenreichii may improve acute colitis by restoring the number of intestinal goblet cells and stimulating the expression of MUC2 in intestinal goblet cells.

What Services Can We Provide for P. freudenreichii at Creative Biolabs?

P. freudenreichii Related Products at Creative Biolabs

  • Strain Products

We supply a variety of P. freudenreichii strains of different preservation numbers as below. If you need other strains, please contact us.

  • Customized strain culture supernatant. (e.g.: for animal research)
  • Customized strain lyophilized powder containing certain CFU. (e.g.: for animal research)

P. freudenreichii displays promising immunomodulatory properties and exert an anti-inflammatory effect via key surface proteins, which have potential for development. Creative Biolabs has 15+ years of experience providing CRO services with a skilled team of scientists. If you are interested in our P. freudenreichii services and products, please contact us for more detail.


  1. Fröhlich, W.M.T.; et al. Cheeses with propionic acid fermentation. Cheese. Academic Press. 2017: 889-910.
  2. An, M.; et al. Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism. Scientific reports. 2021, 11(1): 1-14.

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