Using oxygen-free handling, selective media, and colony-level validation, we isolate and purify B. faecis from complex fecal or biobank samples. Our high-throughput micro-anaerobic screens rank colonies by growth kinetics, SCFA output, and stress tolerance, delivering a panel of high-quality, genetically verified B. faecis strains for downstream discovery and development.
We confirm B. faecis identity via 16S rRNA, ANI-based whole-genome analysis, and metabolic fingerprinting. Deliverables include phylogenetic placement, genome quality metrics, virulence/AMR screens, and curated annotations. This strain-level resolution ensures your B. faecis library is taxonomically sound and functionally informative for comparative studies, repository management, and regulatory-grade documentation.
From bench to pilot, we optimize anaerobic fermentation of B. faecis with media formulation, pH and redox control, gas composition tuning, and carbon-source design to maximize growth and SCFA profiles (acetate, lactate, succinate). We generate scale-down models, DoE datasets, and scale-up parameters suitable for tech transfer and reproducible bioprocessing.
We characterize B. faecis activity through targeted and untargeted metabolomics, epithelial and immune co-cultures, cytokine profiling, and barrier-integrity readouts. Our MoA panel links B. faecis metabolites (e.g., acetate) with effects on inflammatory signaling, tight-junction proteins, and macrophage function to prioritize strains and conditions with consistent, quantifiable bioactivity.
Using polarized Caco-2/HT-29, primary IECs, organoids, and air–liquid interface systems, we assess B. faecis impacts on epithelial pathways, tight junctions (ZO-1, occludin, claudins), and innate signaling. Readouts include TEER, permeability, transcriptomics, and multiplex cytokines, enabling robust translation of B. faecis phenotypes to host-relevant outcomes.
We develop fit-for-purpose stabilization for B. faecis including lyophilization, spray-drying, and microencapsulation. Excipients are screened for anaerobe compatibility, osmoprotection, and reconstitution performance. Stability protocols (ICH-aligned) quantify viability, moisture, and potency under heat, humidity, and oxygen stress to secure shelf-life and supply reliability.
We profile B. faecis safety with whole-genome AMR/virulence scans, hemolysis and cytotoxicity assays, antibiotic susceptibility panels, and endotoxin/impurity checks. Clear, study-ready data packages de-risk research deployment and support internal governance for strain selection, repository control, and onward scale activities.
We map B. faecis responses to oxygen excursions, pH shifts, osmotic and thermal stress. Multi-omics (transcriptome, targeted metabolomics) and growth kinetics identify tolerance thresholds and adaptive signatures, guiding media design, process parameters, and stabilization choices to maintain phenotype and function in real-world handling.
