Creative Biolabs establishes rigorously controlled anaerobic workflows to selectively isolate R. hominis from human fecal samples or existing repositories. Using tailored media, redox control, and colony screening, we enrich for R. hominis while minimizing competing flora. Isolates are evaluated for consistent growth, butyrate output, and genomic stability, enabling you to prioritize the most robust R. hominis strains for downstream experimentation.
To confirm species- and strain-level identity of R. hominis, Creative Biolabs combines 16S rRNA gene sequencing, whole-genome sequencing, and detailed phenotypic profiling. High-resolution genotyping supports discrimination from closely related Roseburia spp., while biochemical and SCFA profiles provide functional context. This integrated identification package safeguards data quality and ensures that every dataset you generate is unambiguously linked to your specific R. hominis strain.
R. hominis is a key butyrate producer that metabolizes complex carbohydrates, including resistant starches, inulin, arabinoxylan, and human milk-derived glycans. Creative Biolabs maps carbohydrate fermentative profiles of R. hominis using defined substrates, time-resolved growth curves, and targeted SCFA quantification (butyrate, propionate, acetate). By linking substrate utilization to metabolite signatures, we help you position R. hominis within diet-microbiome-host interaction frameworks and nutritional intervention concepts.
Our functional and MoA screening platform assesses how R. hominis influences key biological readouts under controlled in vitro conditions. Creative Biolabs quantifies SCFA production kinetics, evaluates barrier-related markers such as tight-junction proteins, and monitors signaling pathways implicated in inflammation and metabolic regulation. These assays deliver mechanistic insight into how specific R. hominis strains shape epithelial responses, immune communication, and host metabolic pathways at the molecular level.
Using advanced co-culture systems, Creative Biolabs investigates direct interactions between R. hominis and host cells. We employ human intestinal epithelial lines, primary cells, immune subsets, and organoid-based models to examine barrier integrity, mucus layer dynamics, and innate and adaptive immune engagement. Readouts can include TEER measurements, mucin expression, pattern-recognition receptor signaling, and microbial adhesion profiles, generating a rich dataset on R. hominis–host cross-talk.
To dissect the immunological footprint of R. hominis, Creative Biolabs runs dedicated immune-modulation assays. We characterize cytokine secretion patterns, TLR/NF-κB signaling, microglial or macrophage activation markers, and T cell–associated readouts in relevant cell systems. These experiments enable you to define immunological signatures associated with R. hominis exposure, supporting research narratives on mucosal homeostasis, systemic inflammation, and gut–brain or gut–lung axes.
Our anaerobic fermentation platform supports research-scale production of R. hominis from shake-flask level to controlled bioreactors. Creative Biolabs optimizes pH, redox potential, agitation, headspace gas composition, and feeding strategies to maximize viable R. hominis biomass while preserving SCFA production profiles. Process data and in-process analytics (CFU counts, optical density, metabolite panels) provide a solid foundation for reproducible preclinical batch generation and process-development studies.
R. hominis is highly oxygen-sensitive, so stabilization is critical for distribution and downstream formulation work. Creative Biolabs designs stabilization strategies tailored to R. hominis, including cryopreservation in optimized cryoprotectants, lyophilization with protective excipients, and packaging compatible with low-oxygen environments. We evaluate post-storage viability, SCFA production, and genomic stability to help you maintain functional R. hominis across storage and transport scenarios.
