Creative Biolabs isolates R. intestinalis from fecal or synthetic community samples using strict anaerobic workflows, selective media, and high-throughput colony screening. R. intestinalis candidates are prioritized based on butyrate production, genomic stability, and phenotypes aligned with your microbiome research objectives.
Our team confirms R. intestinalis identity by 16S rRNA gene sequencing, whole-genome sequencing, and targeted qPCR, supported by curated reference databases. This ensures that R. intestinalis strains entering downstream studies are accurately classified, genetically characterized, and fully traceable for regulatory-ready documentation.
R. intestinalis efficiently ferments complex plant polysaccharides and β-mannans into short-chain fatty acids, especially butyrate. Creative Biolabs quantifies R. intestinalis carbohydrate utilization profiles across tailored fiber panels, SCFA outputs, and pH shifts, providing mechanistic insight into diet–microbe interactions and substrate selection for formulation design.
We design R. intestinalis functional and mechanism-of-action assays to interrogate butyrate production, gene expression changes, and signaling readouts in disease-relevant in vitro systems. Using omics, reporter assays, and immune co-cultures, Creative Biolabs links R. intestinalis exposure to host pathways such as NF-κB, TLR5, tight junction regulation, and T cell modulation.
Our host–microbe interaction tests examine how R. intestinalis affects epithelial barriers, mucus production, and cytokine release. We use transwell intestinal monolayers, co-culture systems, and barrier integrity assays (e.g., TEER, FITC-dextran flux) to quantify how R. intestinalis–derived metabolites and surface components modulate permeability and junctional protein expression.
Creative Biolabs offers in vitro immune assays to measure how R. intestinalis regulates dendritic cells, macrophages, and T cells. We evaluate Treg differentiation, CD8⁺ T cell activation, cytokine profiles, and pattern-recognition receptor signaling using conditioned media, purified metabolites, or R. intestinalis–derived extracellular vesicles in well-controlled co-culture models.
R. intestinalis requires strict anaerobic, fine-tuned fermentation conditions to maintain viability and metabolite output. Creative Biolabs scales R. intestinalis cultures from lab flasks to bioreactors, optimizing parameters such as pH, redox potential, fiber substrates, and gas composition to deliver high-density biomass and consistent butyrate profiles for downstream research.
Our formulation scientists transform R. intestinalis cultures into research-grade preparations suitable for in vivo and in vitro studies. We assess excipients, cryoprotectants, encapsulation strategies, and storage conditions to preserve R. intestinalis viability, genetic stability, and functional traits within your required dosing and handling specifications.
Fig.1 Gram staining of R. intestinalis.
