Operate strictly anaerobic pipelines to recover, purify, and bank A. caccae from complex matrices. High throughput plates and parallel chemostats quantify butyrate outputs, lactate clearance, acetate co utilization, and growth kinetics. We rank strains by butyrate yield, robustness to bile and brief oxygen exposure, and compatibility with lactate providing partners for rational consortia design, and scalable biobanking procedures.
Confirm A. caccae identity and purity with 16S rRNA sequencing, whole genome sequencing, and MALDI-TOF profiles supported by curated reference spectra. Bioinformatics resolves strain level clades, flags contaminant signatures, and screens for safety relevant loci. We document provenance, depositable sequences, and annotated genomes to enable traceable projects and reliable cross-study comparability for partner labs and regulatory audiences.
Develop scalable anaerobic processes from shake flasks to instrumented bioreactors. We optimize carbon sources—lactate with acetate, resistant starches, or inulin—pH control, redox buffers, and gas composition. Online off-gas analytics and GC/LC quantification track SCFA production, enabling fed-batch or continuous runs tailored to target butyrate profiles and downstream formulation requirements, with robust CMC style documentation packages.
Engineer stability for real-world handling. We compare lyophilization and spray drying, low oxygen packaging, antioxidants, and cryo-protectants across excipient matrices. Stability studies quantify viability, butyrate productivity, and moisture over temperature and logistics cycles, selecting robust conditions that preserve functional performance and simplify later scale up, storage, shipping, and shelf-life claims, with clear, reproducible test reports.
Design mono strain or co formulated consortia with lactate supplying partners. We match prebiotic carriers and release strategies, build capsules, granules, or freeze dried powders, and test compatibility. Data verify survival, butyrate output, and functional integrity across blends, excipients, and dosing matrices to bridge bench performance and application relevant specifications, with transparent, formulation ready dossiers.
Build mechanism evidence around SCFA spectra, lactate scavenging, and cross feeding. High content metabolomics, enzyme assays, and flux proxies quantify butyrate pathways and acetate coupling. Stress panels evaluate pH and bile resilience. Results connect strain properties to functional outcomes, supporting prioritization, partner selection, and rational design of synergistic consortia, with reproducible, decision oriented analytics packages.
Quantify barrier and signaling outcomes in mucus enhanced epithelial models and cocultures. TEER, tight junction proteins, and permeability dyes track barrier status. Conditioned media and purified metabolites isolate drivers. Cytokine panels and transcriptomics reveal pathway shifts that link A. caccae fermentation matrices with host relevant phenotypes across realistic exposure conditions, with rigorous controls and baselines.
Interrogate epithelial, macrophage, and dendritic cell models using live cells, conditioned supernatants, and butyrate controls. We quantify IL-8, TNF-alpha, NF-kappaB activity, TEER, and relevant chemokines over dose and time. Data differentiate contact dependent and metabolite mediated effects, supporting precise mechanism narratives and dose matrix guidance for downstream development, with standardized stimuli and orthogonal validation assays included.
