Anaerostipes caccae as Next Generation Probiotics

Anaerostipes caccae (A. caccae) was originally one of the major bacteria that produced butyrate through lactic acid metabolism in the adult gut. It is now considered to have health effects on humans. Creative Biolabs is a multidisciplinary, qualified & experienced team. We are 100% dedicated to live biotherapeutic products (LBP) development. We will work with you as part of your team to provide timely and effective solutions.

Introduction of A. caccae

A. caccae is a member of the Clostridial cluster XIVa Firmicutes bacterial group. The organism was first described in 2002 after being isolated from human feces. A. caccae is an obligate anaerobe. Isolates are Gram variable, non-motile, and rod-shaped bacteria with the ability to produce butyrate from glucose metabolism. Individual cells are 0.5-0.6 µm in width×2.0-4.0 µm in length and occur in chains of up to 4 cells. On Colombian blood agar, it forms white-opaque colonies that are round, smooth, shiny, sticky, and no hemolysis. The strains are catalase and oxidase negative. Butyrate, acetate, and lactate are the main products of glucose metabolism. The DNA G+C content is 45.5-46.0 mol%. The GenBank accession number of the 16S rRNA sequence of the type strain is AJ270487.

Gram stain of <em>A. caccae</em> isolate from blood culture. Fig.1 Gram stain of A. caccae isolate from blood culture. (Workneh, 2016)

A. caccae for Health Benefits

  • Allergic Responses

Microbiome-based treatments can be considered a potential treatment for food allergies. Scientists colonized germ-free mice with feces from healthy or cow’s milk allergic (CMA) infants. They found that germ-free mice colonized with bacteria from healthy, but not CMA, infants were protected against anaphylactic responses to a cow’s milk allergen. Differences in bacterial composition in human donors and transplanted mice separate the healthy population from the CMA population. To further study the role of A. caccae in gene expression regulation, mice were then administered with A. caccae. A. caccae were able to reduce the expression of T2 dependent, antibodies, Acot12 expression as well as cytokine responses IL-13 and IL-4, which promoted oral tolerance towards cow’s milk allergy. This opens new perspectives for the treatment of food allergies in humans.

In recent years, intestinal flora has been found to play an important role in the regulation of anti-cancer immune and therapeutic responses. Scientists selected a mix of four Clostridiales species (CC4), namely Roseburia intestinalis, Eubacterium hallii (Anaerobutyricum hallii), Faecalibacterium prausnitzii, and A. caccae for investigations in murine models of colorectal cancer (CRC). Oral administration of a mixture CC4 in mice could prevent or even successfully treat CRC as an independent treatment. This effect depended on intratumoral infiltration and activation of CD8+ T cells. In a therapeutic setting, CC4, as well as R. intestinalis and A. caccae as individual single strains, were successful in decreasing the CRC tumor burden. Their findings provide a strong preclinical foundation for exploring gut bacteria as novel stand-alone therapy against solid tumors.

Commensal <em>Clostridiales</em> strains mediate effective anti-cancer immune response against solid tumors. Fig.2 Commensal Clostridiales strains mediate effective anti-cancer immune response against solid tumors. (Montalban, 2021)

What Services Can We Provide for A. caccaeat Creative Biolabs?

A. caccae Related Products at Creative Biolabs

  • Strain Products

We supply a variety of A. caccae strains of different preservation numbers as below. If you need other strains, please contact us.

  • Customized strain culture supernatant. (e.g.: for animal research)
  • Customized strain lyophilized powder containing certain CFU. (e.g.: for animal research)

An extensive study of the potential use of selected probiotic bacteria species and their strains is desperately needed for the prevention and treatment of numerous human and animal diseases. In this regard, Creative Biolabs will be your best partner for LBP development. Our experts provide us with the right support and expertise for each of the different stages of the development of our project. Please feel free to contact us for further discussion of A. caccae.

References

  1. Workneh, M.; et al. Bypass graft infection and bacteremia caused by Anaerostipes caccae: First report of human infection caused by a recently described gut anaerobe. Anaerobe. 2016, 42: 98-100.
  2. Montalban, A.A.; et al. Commensal Clostridiales strains mediate effective anti-cancer immune response against solid tumors. Cell host & microbe. 2021, 29(10): 1573-1588. e7.

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