Creative Biolabs isolates high-viability L. crispatus from healthy donor-derived samples using selective culturing and confirmatory identification. Screening prioritizes strains with strong acidification profiles and robust oxidative/antimicrobial signatures in controlled models, building a protected seed-stock library designed for downstream development and reproducibility.
To characterize L. crispatus mechanisms, we quantify acidification kinetics, D-lactate signatures, and secreted antimicrobial activity, then validate competitive exclusion against representative pathobionts using co-culture and community-shift readouts. Mechanistic outputs are aligned to claim-ready endpoints grounded in published BV recurrence and microbiome restoration research.
For L. crispatus, epithelial adherence is a core predictor of persistence. We run in vitro adhesion assays on vaginal or intestinal epithelial models, quantify attachment and biofilm-associated traits, and measure barrier-relevant readouts (e.g., junction integrity markers and innate signaling panels) to show how the strain behaves at the host interface.
Given the documented ability of L. crispatus secreted factors to suppress Candida virulence behaviors in epithelial models, Creative Biolabs offers targeted antifungal testing against Candida albicans, including growth/filamentation endpoints, microscopy-supported morphology scoring, and condition-optimized assays that reflect acidic microenvironment constraints.
L. crispatus often shows stringent growth requirements. We optimize media composition (carbon/nitrogen sources, trace elements) and bioprocess parameters (pH control, temperature, oxygen limitation strategies) for high-density cultivation while preserving viability and functional phenotype, with in-process QC to prevent drift across scale-up stages.
Creative Biolabs develops protective formulations tailored to L. crispatus sensitivity, including freeze-drying protectant systems and delivery-format design (oral capsule concepts vs. vaginal gel/suppository prototypes). Formulation choices are justified by survival-through-processing data and release/rehydration performance under relevant conditions.
Because L. crispatus viability can decline rapidly with moisture/temperature stress, we run accelerated and long-term stability studies across packaging options and storage conditions. Outputs include viable count trajectories, functional retention checks, and shelf-life modeling suitable for internal gating decisions and partner due diligence.
To precisely quantify L. crispatus in complex communities, we design and validate qPCR primers/probes that discriminate L. crispatus from close relatives (e.g., L. iners) using specificity panels and standard curves. These tools support accurate tracking in microbiome studies, challenge models, and validation datasets.
