Creative Biolabs performs targeted isolation of L. plantarum from fermented foods, plant matrices, or human-derived samples, then applies high-throughput phenotypic screening to prioritize wild-type candidates. Each L. plantarum shortlist is built around colonization-relevant traits, signature metabolite profiles, and high tolerance to processing-relevant stresses.
We confirm L. plantarum identity using 16S rRNA sequencing and whole-genome sequencing (WGS), enabling strain-level resolution and genetic background interpretation. For each L. plantarum candidate—natural or engineered—we provide traceable identity reports, safety-oriented genomic checks, and documentation aligned with research compliance expectations for strain handling.
Creative Biolabs engineers L. plantarum as a chassis using synthetic biology strategies that match your expression and stability requirements. We support vector design, genome editing options, and metabolic engineering optimization to build L. plantarum strains intended to express functional proteins/peptides or enhanced pathways under defined culture constraints.
We stress-test L. plantarum across simulated gastric acidity, bile salts, osmotic pressure, temperature shifts, and oxidative exposure—then quantify survival, recovery kinetics, and metabolic output. This creates a practical robustness profile for each L. plantarum strain, helping you select candidates that remain viable and metabolically consistent under harsh, real-world-like conditions.
Creative Biolabs evaluates L. plantarum function with mechanism-anchored assays including mucin/mucus adhesion, pathogen-growth interference readouts, SCFA-related metabolic indicators, and barrier-focused endpoints (e.g., epithelial integrity proxies). Each L. plantarum screen is designed to distinguish strain-specific effects from media artifacts and batch noise.
Using immune-cell co-culture models, we quantify how L. plantarum strains influence cytokine patterns (e.g., IL-10, IL-12, TNF-α) under standardized stimulation settings. These assays help rank L. plantarum candidates by immune-signature directionality and consistency, supporting hypothesis-driven positioning for immunology-facing research programs.
We develop customized fermentation strategies for L. plantarum by optimizing media composition and bioprocess parameters (pH control, dissolved oxygen, feeding strategy, agitation). The goal is to reach high-density, reproducible L. plantarum growth while maintaining plasmid stability (when applicable) and preserving key phenotype markers tied to downstream performance.
Creative Biolabs designs stabilization solutions for L. plantarum, including lyoprotectant screening for freeze-drying and microencapsulation options to improve survival through processing and storage. Each L. plantarum formulation plan is guided by viability kinetics, moisture sensitivity, and intended handling conditions to protect shelf-life consistency.
