Our microbial identification service ensures precise characterization of B. breve strains through a multi-tiered workflow. We integrate anaerobic morphology, biochemical profiling, MALDI-TOF, and advanced sequencing such as 16S rRNA or WGS. This not only confirms taxonomic identity but also detects unique genomic traits such as carbohydrate utilization islands and bile-salt hydrolases. Detailed reports highlight genetic features with potential impact on metabolic activity and host interaction, giving you a data-rich basis for further development and decision-making.
Creative Biolabs specializes in isolating B. breve from complex human or environmental samples under strict anaerobic conditions. After isolation, colonies undergo functional screening for growth kinetics, pH and bile tolerance, and carbohydrate utilization efficiency. Each isolate is benchmarked for robustness and metabolic adaptability. Deliverables include a ranked panel of candidate strains with clear phenotype data, enabling your team to prioritize isolates for downstream applications such as fermentation, immune assays, or synbiotic formulation.
Our functional screening platform captures how B. breve exerts measurable influence on its environment. We quantify short-chain fatty acids (acetate, lactate, propionate), profile exopolysaccharide composition, and evaluate cross-feeding interactions with other microbiota members. This molecular and metabolite-level analysis connects strain-specific outputs to host-relevant functions. By mapping data to mechanism-of-action hypotheses, we help researchers establish clear experimental narratives linking microbial features with biological effects, thus supporting both academic investigation and translational applications.
To explore how B. breve communicates with host immunity, we employ epithelial co-culture, dendritic cell assays, and cytokine panel testing. These models evaluate shifts in IL-10, IL-6, and TNF-α secretion, T-cell polarization, and barrier-related responses. Results highlight strain-dependent immunomodulatory profiles, critical for understanding host-microbe dynamics. Researchers receive comprehensive immune readouts with direct applicability for hypothesis-driven projects focusing on mucosal interactions, allergy models, or inflammation pathways.
Our antimicrobial susceptibility testing follows standardized anaerobe-adapted protocols, determining MIC values against a diverse antibiotic panel. Strain-level differences are carefully recorded, and intrinsic resistances are flagged. Findings are contextualized against published datasets to provide clear interpretation of resistance potential and safety considerations. This testing ensures researchers have complete insight into the genetic and phenotypic traits of their B. breve strains, aiding compliance and research design.
Our fermentation service maximizes B. breve biomass yield without compromising functional stability. We optimize conditions including pH (~6.0–7.0), temperature (~36–38 °C), carbon source, and redox environment. Real-time monitoring ensures high viability and reproducibility across runs. The resulting fermentation batches are suitable for stability assays, immune testing, and pre-formulation development.
Stability testing measures how B. breve performs across acid, bile, oxygen, and temperature challenges. We simulate gastrointestinal transit, conduct long-term storage trials, and assess protective excipients during freeze-drying or spray-drying. Data highlight survival curves, functional retention, and metabolic stability. These insights guide formulation strategies and packaging decisions, ensuring research strains retain reproducibility and performance across varied experimental and storage conditions.
Creative Biolabs' lab-scale production service transitions promising B. breve strains into controlled bioreactors with integrated quality monitoring. We optimize medium formulation, agitation, and anaerobic balance to ensure scalable, reproducible growth. Each batch includes viability, purity, and metabolite assessments. Deliverables cover technical reports, Certificates of Analysis, and recommendations for pilot manufacturing.
