For B. acidifaciens, we optimize strictly anaerobic media, headspace gases (H2/CO2/N2), pH, and carbon flux to stabilize yields from shake-flask to lab/pilot bioreactors. We implement dissolved gas control, redox monitoring, and on-line sampling to track organic acids and BA metabolic fingerprints, enabling predictable scale-up and lot-to-lot consistency.
B. acidifaciens demands protection from oxygen and gastric stress. We design lyoprotectant systems and microencapsulation/embedding options to improve storage and simulated GI transit survival. Our deliverables include viability recovery curves, capsule integrity, disintegration profiles, and compatibility matrices tailored to BA biomass, excipient class, and target delivery route.
We systematically stress-test B. acidifaciens under temperature/relative humidity, oxygen excursions, bile/pH shocks, and osmotic shifts. Outputs include freeze-drying cycle development, cryo/lyo excipient screening, and a validated stabilization protocol. The result is a reproducible BA stability design space that balances viability, metabolic competency, and reactivation kinetics.
We map B. acidifaciens functional outputs across epithelial barrier integrity, NF-κB signaling, GPCR reporter pathways, and metabolite activity (acetate/succinate). Assays quantify dose–response, kinetics, and synergy with fibers or co-cultured taxa, supporting a coherent MoA narrative for candidate BA strains or multi-strain concepts.
Using polarized epithelial monolayers, intestinal organoids, and gut-on-chip, we evaluate B. acidifaciens adhesion, mucus interface behavior, tight-junction modulation, and host transcriptional programs. Readouts include TEER, junctional proteins, RNA-seq signatures, and secretome changes to pinpoint BA–host crosstalk nodes relevant to barrier and immune homeostasis.
We characterize B. acidifaciens utilization of oligosaccharides and dietary fibers, deriving acidification kinetics and short-chain fatty acid (SCFA) output. Substrate–product mapping links acetate and succinate production to feedstocks, informing prebiotic pairing, formulation design, and feeding strategies in advanced in vitro or ex vivo systems.
We quantify B. acidifaciens effects in macrophage, dendritic cell, and epithelial–immune co-cultures: cytokine panels, Treg-associated signaling, and inflammatory pathway modulation. IgA-relevant endpoints and antigen-presenting cell phenotypes are profiled to support immunology-focused go/no-go decisions and ranking across strain or process variants.
We expose B. acidifaciens to oxygen pulses, bile salts, low pH, osmolarity shifts, temperature ramps, and shear. Multi-omics (transcript/metabolite) and viability data identify tolerance thresholds and adaptive programs, de-risking process selection and formulation choices for improved robustness in real-world handling scenarios.
