Bacteroides acidifaciens as Next Generation Probiotics

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Introduction of Bacteroides acidifaciens(BA)

BAcells are obligately anaerobic, non-spore forming, nonmotile, Gram-negative rods. Cells grown on EG agar plates are 0.8~1.3×1.6~8.0μm. Colonies on EG agar plates after two days of incubation are 1~3 mm in diameter, circular, entire, raised convex, smooth, and greyish-colored. The major end products of glucose fermentation are acetic and succinic acids. At the molecular level, this species can be distinguished from other Bacteroides species by the sequence of the 16S rRNA gene. Strain A40T is the type strain and has been deposited in the Japan Collection of Microorganisms as JCM 10556T.

BA for Disease Treatment

As mice aged, scientists found significantly reduced body weight and fat mass in Atg7ΔCD11c mice when compared with Atg7f/f mice. When the mice shared commensal bacteria through cohabitation or fecal transfer experiments, the two groups had similar body weight and fat mass. By pyrosequencing analysis, BA was significantly increased in feces of Atg7ΔCD11c mice compared with those of control Atg7f/f mice. Wild-type C57BL/6 (B6) mice fed with BA were significantly more likely to gain less weight and fat mass than mice fed with PBS. In addition, the serum insulin level of B6 mice fed with BA increased, while the serum glucagon-like peptide-1 increased and the intestinal dipeptidyl peptidase-4 decreased. These findings suggest that BA may be a potent tool for metabolic diseases such as diabetes and obesity.

Proposed models of “lean bug” <em>BA</em> to protect host against insulin sensitivity and obesity. Fig.1 Proposed models of “lean bug” BA to protect the host against insulin sensitivity and obesity. (Yang, 2017)

  • Liver Injury

Scientists found that the gut microbiota from gentamycin (Gen)-treated mice significantly alleviated concanavalin A (ConA)-induced liver injury compared to vancomycin (Van)-treated mice by inhibiting CD95 expression on the surface of hepatocytes and reducing CD95/CD95L-mediated hepatocyte apoptosis. Through the combination of microbiota sequencing and correlation analysis, we isolated 5 strains with the highest relative abundance from the feces of Gen-treated mice. Only BA played a protective role against ConA-induced liver injury. The work showed that a specific murine intestinal bacterial strain, BA, ameliorated liver injury by reducing hepatocyte apoptosis in a CD95-dependent manner. Determination of the function of BA may provide an opportunity for its future use as a treatment for liver disease.

Proposed models of “lean bug” <em>BA</em> to protect host against insulin sensitivity and obesity.Fig.2 Working model of the mechanism of BA protection against CD95-dependent autoimmune/alcohol-induced liver injury. (Wang, 2022)

What Services Can We Provide for BA at Creative Biolabs?

BA Related Products at Creative Biolabs

  • Strain Products

We supply a variety of BA strains of different preservation numbers.

  • Customized strain fermentation supernatant. (e.g.: for animal research)
  • Customized strain lyophilized powder containing certain CFU. (e.g.: for animal research)

Creative Biolabs specializes in the LBP development of different next generation probiotic candidates. We have unique R&D expertise to provide the highest quality custom LBP services and products. If you are interested in our BA related services or products, please feel free to contact us for more.

References

  1. Yang, J.Y, et al. Gut commensal Bacteroides acidifaciens prevents obesity and improves insulin sensitivity in mice. Mucosal immunology. 2017, 10(1): 104-116.
  2. Wang, H.; et al. Bacteroides acidifaciens in the gut plays a protective role against CD95-mediated liver injury. Gut microbes. 2022, 14(1): 2027853.

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