Engineering Bacillus Services for Live Biotherapeutics Drug Discovery
Background Applications Services Advantages FAQs Resources
Background
Bacillus is a group of Gram-positive, endospore-forming bacteria species, which has multiple functions and is one of the most promising industrial microorganisms. Over the years, transgenic Bacillus subtilis and other Bacillus species have been used in a wide range of applications ranging from pharmaceutical proteins (antibody fragments, interleukin and interferon, hormone precursors, growth factors, and antimicrobial peptides) to the heterogeneous production of industrial and food-grade enzymes.
Biotechnological Versatility of Bacillus spp.
Bacillus has become an important platform for producing various enzymes and chemicals. Multiple peptides, growth factors, enzymes, vitamins, amino acids, and low molecular weight compounds have been expressed in recombinant Bacillus subtilis. Recombinant strains of Bacillus subtilis have even entered the field of immunology and become an important component of vaccines.
Fig.1 Biotechnological versatility of Bacillus spp.1
Genetic Engineering in Bacillus spp.
Genetic improvement of strains involves the development of a range of molecular application methods. In recent years, the bioengineering of Bacillus has changed very greatly, with the knockout and overexpression of multiple genes enabling the manipulation of entire metabolic pathways. The gene-editing system is the latest advance in the field of genome editing, and it has become a state-of-the-art genome editing tool that is efficient, versatile, and easy to use. The vectors used in Bacillus mainly include two types: autonomic replication and integration. These vectors contain several required genetic regions: one or more promoters, regulatory regions, specific sites where replication occurs, and one or more genes that allow for selective recombination. In recent years, Bacillus vectors with Cas9 and necessary auxiliary sequences (such as sgRNA, and single guiding RNA) have become very popular. Advances in genetic engineering strategies have enabled the potential of Bacillus as a productive host to be realized and put Bacillus in competition with conventional industrial microorganisms.
Fig.2 Genetic engineering in Bacillus spp.1
Engineering Bacillus Services
How Bacillus spp. Strain Construction Service Can Assist Your Project
Bacillus spp. strain construction services can significantly enhance your project by providing customized microbial strains tailored to your specific needs.
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Strain Optimization: custom strains can be optimized for higher expression levels of target proteins, enzymes, or metabolites.
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Genetic Modifications: utilizing gene-editing tools, strains can be engineered to include or delete specific genes, enhancing desired traits.
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Improved Secretion Pathways: enhancing protein secretion pathways to facilitate easier purification and higher yields.
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Stress Resistance: engineering strains to withstand various environmental stresses, making them more robust for industrial applications.
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Safety and Compliance: developing non-pathogenic strains that comply with regulatory standards for use in food, pharmaceuticals, and other industries.
These services can streamline your research and development process, ensuring you have the most efficient and effective microbial strains for your project.
Advantages
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Short fermentation cycle.
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Ease of cultivation, robust growth.
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Non-pathogenic, Generally Regarded as Safe (GRAS) status.
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The ability to secrete recombinant proteins in the medium.
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The lack of external endotoxins.
Frequently Asked Questions
What Types of Researchers are Involved in Bacillus spp. Recombinant Strain Construction Services?
Researchers involved in
Bacillus spp. recombinant strain construction comes from diverse fields, including:
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Microbiologists: They study the physiology, genetics, and behavior of microorganisms, including Bacillus species.
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Molecular Biologists: They focus on manipulating genetic material to create recombinant strains.
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Bioengineers: They apply engineering principles to biological systems to optimize strain performance for industrial applications.
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Biotechnologists: They work on developing and applying biological organisms or systems to create products and technologies.
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Geneticists: They study genes and genetic variations to understand how traits are inherited and expressed.
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Bioinformaticians: They use computational tools to analyze genetic data and support strain construction efforts.
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Industrial Scientists: They focus on scaling up and optimizing production processes for commercial applications.
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Pharmaceutical Scientists: They develop therapeutic applications and investigate the potential health benefits of recombinant strains.
These experts collaborate to design, develop, and optimize recombinant strains of
Bacillus spp. for various applications, including enzyme production, biocontrol agents, probiotics, and more.
Resources
Creative Biolabs has extensive experience in the field of probiotics, as well as an advanced genetic engineering platform, focusing on providing customized projects for customers. If you are working on a specific application or project that could benefit from these services, please feel free to contact us for more.
Reference
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Arsov, Alexander, et al. "Cloning Systems in Bacillus: Bioengineering of Metabolic Pathways for Valuable Recombinant Products." Fermentation 10.1 (2024): 50. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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