Engineering Bacillus licheniformis Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
Bacillus licheniformis (B. licheniformis) is a unique species of organism in the genus Bacillus. It is a Gram-positive bacterium that produces endospores and can produce a variety of antibacterial substances. This bacterium can be used to treat ecological disorders caused by various diseases, and it has a very effective bacterial antagonism system that produces antimicrobial peptides with different structures, targeting a variety of bacterial and fungal representatives, including animal and human pathogenicity, and is considered a promising probiotic. In addition, as a probiotic, it can also produce a large number of substances with antioxidant and immunomodulatory activities. At present, the use of probiotics and their metabolites is also considered a new way to control and prevent various infectious diseases. In addition, bacteriocins in B. licheniformis are considered potential natural agents for food preservation.
Current technology allows strains of B. licheniformis to be constructed to produce multiple antimicrobial peptides or proteins, or combinations thereof, that target specific pathogens. It plays an important role in the field of biotechnology as an expression platform, compound producer, environmental application bacteria, and probiotics. Applications in the field of biotechnology include human health, veterinary applications, and aquaculture, which can be used alone or in combination with other probiotic strains.
Fig.1 Mechanisms of action reported for B. licheniformis.1, 4
Engineering B. licheniformis Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain.
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Target gene name.
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Target gene sequence. (if the whole genome sequence is unavailable.)
Advantages
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B. licheniformis can produce a large number of substances with antimicrobial activity of different structures.
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Some strains of B. licheniformis can act as natural carriers of antibiotic substances, fighting various types of pathogens in true probiotic form.
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Intestinal localization of B. licheniformis protects the secretion of active substances from the gastric environment.
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B. licheniformis is a probiotic that is safe to eat, with the ability to resist conditions throughout the gastrointestinal system due to its ability to form spores.
Frequently Asked Questions
Can B. licheniformis be used as a probiotic?
Yes,
B.
licheniformis can be used as a probiotic. It has shown great potential in promoting gut health, enhancing the immune system, and inhibiting harmful pathogens. Here are some key benefits:
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Gut Health: B. licheniformis supports a healthy gut microbiota, aiding in digestion and nutrient absorption.
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Immune Boost: It helps enhance the immune response, offering protection against infections.
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Antimicrobial Properties: Produces compounds that inhibit the growth of harmful bacteria, ensuring overall health.
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Animal Health: Used in livestock to improve gut health, growth performance, and immunity.
Its resilience in various conditions and its ability to produce beneficial compounds make it a strong candidate for both human and animal probiotic applications.
What are the genetic modification tools for B. licheniformis?
There are several genetic modification tools available for
B.
licheniformis:
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Gene-editing: This system allows for precise genome editing by creating double-strand breaks at specific locations in the DNA.
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It's highly efficient and versatile, making it a popular choice for genetic modifications in B. licheniformis.
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Homologous Recombination (HR): This method relies on the natural ability of B. licheniformis to incorporate exogenous DNA through homologous recombination.
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It's useful for targeted gene insertions and deletions.
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Transposon Mutagenesis: This technique uses transposable elements to create random mutations, which can help identify gene functions and regulatory elements.
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Site-Directed Mutagenesis: Techniques like PCR-based methods are used to introduce specific mutations at particular sites in the genome.
These tools enable researchers to study gene functions, improve metabolic pathways, and develop
B.
licheniformis as a microbial cell factory for producing valuable enzymes, vitamins, and antibiotics.
Published Data
Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion2, 4
Abstract
To facilitate genome editing, the researchers developed a system based on RecT conditional expression that uses rhamnose-induced promoters (Prha) to evaluate the effects of the system by deleting the amyL gene that codes for alpha-amylase, identifying optimal conditions for genome editing. This study has made great progress in improving the recombination efficiency of B. licheniformis by using the RecT-based recombination system. In addition, it provides an efficient genome editing tool for genetic engineering applications of this strain.
Fig.2 Editing of the target gene in the B. licheniformis chromosome using a controllable recombinase system.2, 4
Resources
Creative Biolabs is committed to being your professional partner for research excellence, providing customized services for institutions seeking research and development services. We believe that our team of experts with multidisciplinary professional backgrounds will answer your questions. Please do not hesitate to contact us at any time, we would like to help you.
References
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Ramirez-Olea, Hugo, Bernardo Reyes-Ballesteros, and Rocio Alejandra Chavez-Santoscoy. "Potential application of the probiotic Bacillus licheniformis as an adjuvant in the treatment of diseases in humans and animals: A systematic review." Frontiers in Microbiology 13 (2022): 993451.
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Xue, Fang, et al. "Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion." AMB Express 13.1 (2023): 89.
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Xiao, Fengxu, et al. "Advancing Bacillus licheniformis as a Superior Expression Platform through Promoter Engineering." Microorganisms 12.8 (2024): 1693.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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