Engineering Lactobacillus acidophilus Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
With years of experience in probiotic research, Creative Biolabs has developed a cost-effective engineered strain construction platform to provide our customers with high-quality engineered strains. We provide guaranteed services, starting with our vector optimization and all the way to strain sequencing, to ensure that engineered strains are correctly constructed.
Background
Lactobacillus acidophilus (L. acidophilus) is one of the most widely used probiotics in food. Specific strains such as L. acidophilus NCFM have been designed for biotherapeutic drug development, its intrinsic ability to survive in the gut, coupled with its ease of industrial production and genetic manipulation, has generated interest in developing this strain into a design probiotic that can be enhanced, and it forms an ideal chassis for oral mucosal vaccines and biotherapeutic agents. The first generation of L. acidophilus genome manipulation systems was based on single-cross homologous recombination driven by conditional plasmid replication to achieve target gene knockout. Subsequently, scientists developed a marker-free gene replacement system. Performing gene editing-based genome editing in L. acidophilus and other species lacking a complete gene editing system will require heterologous gene editing expression systems. Scientists reported the development of a Lactobacillus SpyCas9-based genome editing toolkit for programmable editing in L. acidophilus, which could also be broadly applicable to other Lactobacillus species that promote health and are industrially relevant. The use of functional gene editing plasmid delivery is critical, particularly in LABs that lack an active gene editing system, such as L. acidophilus.
Fig.1 Modifications of Cas9 in genome editing.1
Engineering Lactobacillus acidophilus Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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PAGE
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or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain
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Target gene name
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Target gene sequence, if the whole genome sequence is unavailable
How L. acidophilus Strain Construction Service Can Assist Your Project
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Construct the target strains according to the needs of clients or refer to the published scheme.
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Optimize the program to improve the success rate.
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Delivery of the constructed target strains (knockout or integrated).
Advantages
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The technique of producing superior strains through gene editing-based genome editing has captured the interest of a huge number of researchers.
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gene editing-based genome editing technology has less operational difficulty, low editing cost, and higher targeting efficiency.
Frequently Asked Questions
What are the genome editing tools that have been reported for L. acidophilus strains?
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Genome Editing Tools
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Advantages
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Homologous recombination using the pORI system
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Does not depend on transformation efficiency.
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Enables the growth of engineered strains at suitable conditions.
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Enables efficient recovery of stable integrants.
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Applicable to deleting any non-essential genes in a wide range of species.
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pTRK system
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Independent of transformation efficiency.
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upp can be used as a counter-selectable marker for marker-less gene replacement for the positive selection of double recombinants.
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gene editing Nickase-assisted plasmid
toolbox (pLCNICK)
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Gene deletion and insertion are performed using the Cas9D10A (nickase).
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Can avoid DSB-induced lethality, which may be due to the different pathways of nicks.
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Marker free.
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Selection/counter-selection marker system
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Efficient deletion or integration of genes at specific sites.
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The upp counter selectable marker is recyclable.
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Resulting in transgenic or mutant strains do not contain any selectable markers or residual plasmids.
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Stable double-crossover mutants can be constructed.
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Published Data
Genetic engineering of UV-mutated Bifidobacterium longum and L. acidophilus concerning folic acid and Anti-inflammatory productivity
Abstract
Both Bifidobacterium longum and L. acidophilus have anti-inflammatory properties. This study aimed to genetically increase folate production by UV irradiation in two lactic acid bacteria. Bifidobacterium longum and L. acidophilus have been genetically modified for their folate production and anti-inflammatory activity. These four mutants also exhibited anti-inflammatory properties by inhibiting COX-1 and COX-2 activity at higher levels than their wild-type strains and Celecoxib.2
Resources
CreativeBiolabs has more than 10 years of experience in pre-clinical contract research and development services, and our team has extensive expertise in live biotherapeutics development, solving complex research problems by delivering customized solutions. We are a trusted partner in all stages of preclinical development. If you are interested in our engineered Lactobacillus acidophilus service, please contact us to schedule a consultation, we can't wait to hear from you.
References
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Mu, Yulin, et al. "Development and applications of gene editing-based genome editing in Lactobacillus." International Journal of Molecular Sciences 23.21 (2022): 12852.
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Abdel Ghany Elrahmany, Hossam M., et al. "Genetic engineering of UV-mutated Bifidobacterium longum and Lactobacillus acidophilus in relation to folic acid and Anti-inflammatory productivity." Egyptian Journal of Chemistry 66.13 (2023): 983-992.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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