The gut microbiota is a dynamic ecosystem with a profound impact on host health. Understanding its function requires a deep dive into the small molecules—the metabolites—that mediate its interactions with the host. Our Comprehensive Metabolomic Profiling Service offers an unparalleled view into this intricate biochemical landscape, providing the critical data needed to drive your research forward. Whether you are developing novel probiotics, investigating drug-microbiota interactions, or exploring the metabolic basis of disease, our end-to-end service provides the precision, depth, and expertise you need to succeed.
Overview
Our service provides a global, untargeted analysis of the small molecule metabolites in your biological samples. By employing a multi-platform approach, we ensure broad coverage of diverse chemical classes, including short-chain fatty acids (SCFAs), bile acids, lipids, amino acids, and other gut-derived metabolites. We go beyond simple data generation, offering comprehensive data analysis and biological interpretation to help you translate raw data into actionable insights.
Our Comprehensive Scope of Services
We provide both broad discovery-based and highly targeted quantitative analyses to meet the diverse needs of your research project, from initial hypothesis generation to biomarker validation.
Workflow: From Sample to Insight
Our robust, end-to-end workflow ensures high-quality data and meaningful biological insights.
Service Details
Targeted Metabolomic
Deliverables
Untargeted Metabolomic Profiling (Discovery-Based)
This service is designed for exploratory research where the key metabolites of interest are not yet known. We aim to detect and relatively quantify as many metabolites as possible in your samples, providing a global view of the gut metabolome.
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Technology: High-resolution Liquid Chromatography-Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Mass Spectrometry (GC-MS/MS).
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Purpose: Biomarker discovery, identifying novel metabolic pathways, and generating hypotheses for future research.
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Advantages: Broad coverage, no prior knowledge of metabolites required, ideal for complex, multi-group studies (e.g., disease vs. healthy).
Targeted Metabolomic Profiling (Quantitative)
This service focuses on the precise and absolute quantification of specific, pre-defined classes of metabolites known to be highly relevant to the gut microbiome. This is ideal for hypothesis-driven studies where you need accurate and reproducible data on key molecules.
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Technology: Quantitative LC-MS/MS and GC-MS/MS using stable isotope-labeled internal standards.
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Purpose: Validating biomarkers, assessing the efficacy of interventions (e.g., prebiotics or probiotics), and deeply profiling specific metabolic pathways.
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Detailed Panels Include:
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Short-Chain Fatty Acids (SCFAs): Absolute quantification of acetate, propionate, butyrate, valerate, and branched-chain SCFAs (isobutyrate, isovalerate). These are crucial for gut barrier integrity, inflammation, and energy metabolism.
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Primary and Secondary Bile Acids: Profiling of a comprehensive panel of over 20 bile acids, including primary (e.g., cholic acid) and secondary (e.g., deoxycholic acid) bile acids, as well as their glycine and taurine conjugates. This is vital for understanding the enterohepatic circulation and microbial bile acid biotransformation.
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Tryptophan Metabolites: Analysis of the kynurenine pathway (e.g., kynurenine, quinolinic acid) and indole derivatives (e.g., indole-3-acetic acid, indole-3-propionic acid), which are linked to the gut-brain axis, immune function, and gut barrier regulation.
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Neuroactive Metabolites: A targeted panel for neurotransmitter precursors (e.g., GABA, serotonin), short-chain fatty acids, and other microbial products implicated in the gut-brain axis.
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Custom Panels: Development of bespoke panels to meet specific research requirements.
Sample Information & Turnaround Time
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Sample Type
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Recommended Amount
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Turnaround Time (Tentative)
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Feces
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> 300 mg (wet weight)
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9-11 weeks
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Plasma/Serum
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> 300 µL
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7-9 weeks
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Urine
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> 300 µL
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7-9 weeks
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Tissue
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> 200 mg
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9-11 weeks
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Other
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Please inquire
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Please inquire
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Note:
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Turnaround times may vary depending on the project scope, sample number, and requested analysis type.
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Detailed sample collection protocols are available upon request.
Deliverables
You receive a comprehensive final report, including:
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A detailed summary of the methods used.
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Raw and processed data files.
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Statistically significant metabolite lists.
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Pathway analysis results with illustrative figures.
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An executive summary with key biological insights and interpretation by our PhD-level scientists.
Ready to start your Metabolomic project? Click here to request a quote and consultation!
Why Choose Us?
Deep Expertise
Our team comprises Ph.D. scientists with extensive experience in metabolomics, mass spectrometry, and gut microbiome research.
State-of-the-Art Technology
We use the latest high-resolution mass spectrometry platforms from leading manufacturers, ensuring high accuracy and sensitivity.
Customization
We offer flexible solutions, from single-panel targeted analyses to full-scale multi-omics projects, tailored to your specific research goals and budget.
Comprehensive Support
From initial consultation to final data interpretation, we act as a dedicated partner, guiding you through every step of the process.
Integrated Multi-Omics
We provide seamless integration of metabolomics data with 16S rRNA gene sequencing or shotgun metagenomics, offering a holistic view of the microbiome.
Applications
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Drug & Therapeutic Development: Assessing the impact of new drugs on microbial metabolism and identifying microbial-derived biomarkers for therapeutic response.
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Prebiotic & Probiotic Studies: Quantifying the functional output of microbial interventions and confirming the presence of beneficial metabolites.
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Nutritional & Dietary Research: Investigating how different diets or nutritional supplements alter the host-microbe metabolome.
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Biomarker Discovery for Diseases: Identifying metabolite signatures in stool or blood that are predictive of conditions like IBD, diabetes, obesity, and neurological disorders.
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Host-Microbe Interaction Studies: Elucidating the mechanisms by which microbial metabolites modulate host metabolic pathways and immune responses.
Discover the metabolic secrets of the gut microbiome to accelerate your research. Ready to Begin Your Research? Contact us today to schedule a free consultation with our team and discover how our services can accelerate your gut microbiome research.
Frequently Asked Questions (FAQs)
Can you handle frozen fecal samples?
Yes. We have established protocols for the collection, shipping, and storage of frozen fecal samples to maintain metabolite integrity.
What is the difference between targeted and untargeted metabolomics?
Untargeted is a discovery-based approach that aims to detect all possible metabolites without prior knowledge, while targeted focuses on the precise quantification of a pre-defined set of known metabolites.
Can you integrate your metabolomics data with my existing 16S sequencing data?
Absolutely. Our bioinformatics team specializes in multi-omics integration to provide a comprehensive analysis that links microbial taxa with their functional metabolic output.
What kind of data will I receive?
We provide a full report including raw data files, quantified metabolite lists, statistical analyses, pathway maps, and a written interpretation of the findings from our expert scientists.
