Engineering Bifidobacterium longum Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
Bifidobacterium longum encodes multiple immune systems with large and diverse arrays of repeat intervals, suggesting that these systems may be active and protect against invading DNA. Its characterization of the immune system offers a potential opportunity to develop genome-editing tools for next-generation probiotics using endogenous systems.
As a traditional probiotic, engineered Bifidobacterium has also been widely used in recent years. Bifidobacterium longum is an anaerobic bacterium that can selectively locate and proliferate in several mouse solid tumors after systemic application. It is an attractive and safe tumor-targeting vector and a potential anticancer drug that can be effective in the specific treatment of solid tumors. It has been reported that Bifidobacterium longum is modified to express single-stranded variable segments that bind to receptors on the surface of cancer cells and inhibit the growth and proliferation of cancer cells.
In addition, researchers also utilized Bifidobacterium longum to express antioxidant enzymes, anti-inflammatory cytokines, and hormones for anti-inflammatory diseases. To alleviate metabolic disorders, Bifidobacterium longum has also been genetically engineered to express hormones or their derivatives initially released from the intestine to promote drug administration. These engineered probiotics can improve the absorption of nutrients and provide a new method for the treatment of diabetes and obesity.
Engineering Bifidobacterium longum Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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PAGE
or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain.
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Target gene name.
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Target gene sequence. (if the whole genome sequence is unavailable.)
Advantages
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Its known safety profile makes it a reliable choice for genetic engineering.
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Modified Bifidobacterium longum strains can be designed to deliver therapeutic proteins, modulate the immune system, or target specific diseases, expanding their use beyond traditional probiotic roles.
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Bifidobacterium longum can be easily genetically modified using tools, allowing for precise alterations to its genome.
Frequently Asked Questions
How Bifidobacterium longum strain construction service can assist your project?
Bifidobacterium longum strain construction services can significantly enhance your project by providing customized microbial strains tailored to your specific needs. Here are some ways these services can assist:
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Strain Optimization: Custom strains can be optimized for higher expression levels of target proteins, enzymes, or metabolites.
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Genetic Modifications: Strains can be engineered to include or delete specific genes, enhancing desired traits.
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Improved Secretion Pathways: Enhancing protein secretion pathways to facilitate easier purification and higher yields.
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Stress Resistance: Engineering strains to withstand various environmental stresses, making them more robust for industrial applications.
These services can streamline your research and development process, ensuring you have the most efficient and effective microbial strains for your project.
What are the research applications of Bifidobacterium longum genetic engineering strains?
The research and application of
Bifidobacterium longum genetic engineering strains are diversified. Here are some key areas:
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Probiotic Development: Enhancing probiotic properties.
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Therapeutic Applications: Engineering strains to produce therapeutic molecules, such as anti-inflammatory compounds, for treating conditions like inflammatory bowel disease (IBD).
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Disease Prevention: Developing strains that can modulate the gut microbiota to prevent or mitigate diseases, including metabolic disorders and infections.
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Aging Research: Investigating the role of Bifidobacterium longum in promoting longevity and protecting against age-related conditions.
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Environmental Health: Using engineered strains to degrade environmental pollutants and improve bioremediation processes.
These applications highlight the potential of genetically engineered
Bifidobacterium longum strains to contribute to health, nutrition, and environmental sustainability.
Published Data
An oral WT1 protein vaccine composed of WT1‑anchored, genetically engineered Bifidobacterium longum allows for intestinal immunity in mice with acute myeloid leukemia1
Abstract
Wilms' tumor 1 (WT1) is a promising tumor-associated antigen for tumor immunotherapy. Researchers have developed an oral protein vaccine platform composed of genetically engineered Bifidobacterium longum anchored to WT1 and tested its anti-cancer activity in mice. This study provides insights into a novel cancer immunotherapy based on gut bacteria mediated by gut immunity. The immune mechanism of oral anti-cancer vaccine based on Bifidobacterium longum triggering intestinal immunity in mice was elucidated, and the immune cells in the immune system were identified. This oral WT1 protein vaccine has potential advantages such as high safety, low cost, and easy mass production, and is expected to provide good compliance for cancer patients.
Resources
Creative Biolabs has rich project experience in the field of probiotic research, advanced technology platforms, and flexible customized solutions. Please feel free to contact us for more service details, quotation proposals, or even just to discuss the projects.
Reference
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Nakagawa, Natsuki, et al. "An oral WT1 protein vaccine composed of WT1-anchored, genetically engineered Bifidobacterium longum allows for intestinal immunity in mice with acute myeloid leukemia." Cancer Immunology, Immunotherapy 72.1 (2023): 39-53. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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