Looking to speed up your search for probiotics that fight obesity? Tired of slow progress and struggling to find the right bugs and figure out how they work? Creative Biolabs' Probiotic Anti-Obesity Assays can help you move things along faster. We use smart lab tests and animal studies to get you solid info and point you to the probiotics that show potential. Request a Quote
In Vitro Assays
Evaluate the impact of probiotics or their components on the cellular and biochemical processes related to obesity.
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Assessment of Lipid Metabolism
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Inhibition of Lipase Activity.
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Adipocyte Differentiation Assays.
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Lipogenesis and Lipolysis Gene Expression: Analyzing the expression levels of genes treated with probiotics.
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Quantification of Intracellular Triglycerides.
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Fatty Acid Binding Assays: Assessing the capacity of probiotics to bind to fatty acids in vitro.
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Evaluation of Gut Microbiota Modulation
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Assessment of Gut Barrier Function
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Evaluation of Anti-inflammatory and Other Metabolic Effects
In Vivo Assays (Animal Models)
Typically rodents (mice or rats) are fed a high-fat diet, to evaluate the anti-obesity effects of probiotics in a complex biological system.
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Body Weight and Fat Mass Measurement
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Regular monitoring of body weight gain.
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Measurement of fat mass at the end of the study.
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Metabolic Parameters Assessment
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Food Intake and Energy Expenditure.
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Glucose Tolerance Tests (GTT) and Insulin Tolerance Tests (ITT).
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Lipid Profile Analysis.
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Liver Function Tests.
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Gut Microbiota Analysis
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Inflammatory Markers and Adipokine Levels
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Measuring circulating levels of pro-inflammatory cytokines and anti-inflammatory cytokines.
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Assessing the levels of adipokines.
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Histological Analysis
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Examining tissue samples under a microscope to assess adipocyte size, inflammation, and other morphological changes.
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Fecal Metabolite Analysis
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Analyzing the composition of fecal metabolites, including SCFAs.
Turnaround Time
The typical timeframe for this service ranges from 8 to 16 weeks, depending on the specific assays required, the number of probiotic strains being evaluated, and the complexity of the in vivo studies.
What exactly is a probiotic anti-obesity activity assay?
This could involve seeing if they can reduce fat accumulation, improve metabolism, mess with appetite signals, or even change the gut microbiome in a way that's beneficial for weight management.
What kind of probiotic strains can you test?
Pretty much any strain you're interested in! Whether it's a well-known
Lactobacillus or
Bifidobacterium species or something a bit more unusual you've isolated, we can work with it.
What kind of 'activities' do you measure?
This depends on what you're looking for, but some common things we assess are
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Lipase inhibition: Seeing if the probiotics can block enzymes that digest fat.
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Cholesterol reduction: Checking if they can lower cholesterol levels.
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Adipocyte differentiation: Looking at whether they can inhibit the formation of fat cells.
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Gut microbiota modulation: Analyzing changes in the gut bacteria composition after probiotic treatment.
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Production of short-chain fatty acids (SCFAs).
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Effects on inflammatory markers.
We can tailor the assays to fit your specific research goals.
What kind of samples do I need to send you?
Usually, we'll need a pure culture of your probiotic strain.
How much does this kind of testing cost?
The cost depends on the number and complexity of the assays you need.
