Discovering new antifungal drugs is a critical challenge due to rising drug resistance and a limited pipeline of new treatments. Creative Biolabs' High-Throughput Antifungal Hit Discovery & Screening Services are designed to accelerate your research by efficiently identifying promising new compounds. We provide a comprehensive platform, from initial screening to detailed mechanism of action studies, to help you find effective antifungal agents faster. Request a quote

Fig.1 Identification of targets. (Creative Biolabs Authorized)

Overview

We offer a high-throughput screening (HTS) platform that allows for the rapid testing of large chemical libraries against various fungal pathogens. This service is ideal for pharmaceutical companies, academic institutions, and biotechnology firms seeking to discover novel antifungal compounds. Our approach is flexible, allowing us to screen for compounds with different mechanisms of action, including those targeting essential cellular processes or disrupting cell wall and membrane integrity.

Detailed Scope of Services

Our service package is customizable to fit your specific research needs.

Workflow

Fig.2 Workflow for antifungal hit screening. (Creative Biolabs original)

Service Details

Services
Samples
Deliverables
Turnaround Time

Services

  • Assay Development: We can develop and optimize a custom HTS assay for your specific target or pathogen of interest.
  • Primary Screening: Rapid and cost-effective screening of diverse compound libraries against your chosen fungal strain.
  • Dose-Response & IC50 Determination: Confirmation of hit compounds and determination of their half-maximal inhibitory concentration.
  • Secondary Screening: Follow-up assays to confirm activity and explore different mechanisms of action.
  • Selectivity Profiling: Testing the identified hits against mammalian cell lines to assess cytotoxicity and therapeutic index.
  • Time-Kill Assays: Evaluating the rate and extent of fungal killing to determine if the compound is fungicidal or fungistatic.
  • Synergy Studies: Investigating the potential for combining new hits with existing antifungals to overcome resistance.

Sample Information

  • Fungal Strains: We can work with a wide range of clinically relevant fungal species, including Candida spp., Aspergillus spp., Cryptococcus spp., and others. You may provide your strains, or we can use our in-house collection.
  • Compound Libraries: You can provide your chemical library, or you can leverage our extensive collection of diverse, drug-like compounds. Libraries can range from tens of thousands to millions of compounds.

Deliverables

  • Comprehensive Project Report: A detailed document summarizing the screening process, data analysis, and results.
  • Raw Data: All raw assay data files for your analysis.
  • Validated Hit List: A list of confirmed active compounds with their IC50 values and relevant data.
  • Biological Samples: We can provide you with the confirmed hit compounds for further in-house testing.

Turnaround Time

The turnaround time for our services varies depending on the scope of the project, including the size of the compound library and the complexity of the assays. A typical primary screen of 100,000 compounds can be completed in approximately 4-8 weeks from the start of the project. A detailed timeline will be provided after the initial consultation.

Ready to discuss your project? Let us get in touch and explore how our consulting services can help you achieve your goals.

The Necessity of High-Throughput Antifungal Screening

Antifungal resistance is a growing global health threat. Existing drugs often have side effects or limited efficacy; discovering new drug classes is a top priority. Traditional screening methods are slow and labor-intensive. Our HTS platform addresses this need by dramatically increasing the speed and efficiency of the drug discovery process, enabling the testing of millions of compounds in a fraction of the time. This helps to overcome the bottleneck in early-stage drug discovery and accelerates the identification of lead compounds.

Mechanism of Action (MoA) Screening

Understanding a compound's mechanism of action is crucial for its development. We don't just identify hits; we help you understand how they work. Our services include:

  • Cell Wall Integrity: Screening for compounds that inhibit the synthesis or proper function of the fungal cell wall.
  • Cell Membrane Disruption: Identifying agents that target ergosterol synthesis or otherwise compromise the fungal cell membrane.
  • Inhibition of Key Enzymes: Assays targeting specific enzymes essential for fungal survival, such as β-1,3-glucan synthase.
  • Nucleic Acid Synthesis Inhibition: Screening for compounds that interfere with fungal DNA or RNA replication.

Advantages of Partnering with Creative Biolabs

Speed & Efficiency

Our automated HTS platform significantly reduces the time and cost associated with early-stage drug discovery.

Expertise

Our team consists of experienced microbiologists, biochemists, and screening experts.

Flexibility

We offer a customizable approach, from assay development to bespoke screening cascades.

State-of-the-Art Technology

We utilize advanced robotics and plate readers to ensure high-quality, reproducible data.

Comprehensive Support

We provide end-to-end support, from project design to data interpretation.

Fig.3 Target validation. (Creative Biolabs Authorized)

Applications

  • New Drug Discovery: Identifying novel antifungal agents with new mechanisms of action.
  • Repurposing Existing Drugs: Screening existing approved drugs for new antifungal activity.
  • Academic Research: Supporting academic labs in their fundamental research on fungal biology and pathogenesis.
  • Agrochemicals: Discovering new fungicides for agricultural applications.

Your next breakthrough is just a conversation away. Let's talk about your challenges and find a custom solution together.

FAQs

Can you screen my compound library?

Yes, we are fully equipped to screen a library provided by your company or institution.

What is the minimum number of compounds I can screen?

We can accommodate projects of various sizes, from hundreds of compounds to tens of thousands of compounds.

How do you handle intellectual property (IP)?

We prioritize the protection of your IP. All projects are conducted under a strict confidentiality agreement.

What if I don't have a specific assay in mind?

Our team can help you design and optimize a suitable assay based on your research objectives.

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