Comprehensive Probiotic Anti-Infection Assay

Probiotic Characterization

• Strict quality control inspections are carried out to ensure: vitality, purity, and stability.
• High-quality probiotic cultures ready for in vitro and in vivo testing.

In Vitro Screening Assays

• Antimicrobial Activity Assays: Test the inhibitory or killing effects of probiotics on the growth of various pathogenic bacteria.
  
  • Agar Diffusion Tests: Assessing the ability of probiotics or their supernatants to inhibit the growth of pathogenic microorganisms on agar plates by measuring zones of inhibition.
  • MIC and MBC Assays: Determining the lowest concentration of a probiotic or its antimicrobial substances required to inhibit or kill a specific pathogen in liquid culture.
  • Time-Kill Kinetics Studies: Evaluating the rate and extent of pathogen reduction over time when exposed to probiotics or their metabolites.
  • Antifungal Activity Assays: Specific tests to determine the inhibitory effects of probiotics against fungal pathogens.
• Anti-Viral Activity: Evaluate the inhibitory effect of probiotics on the growth or infection of viruses.
• Adhesion Assays: Assessment of probiotic adherence to epithelial cells.
• Anti-Biofilm Assays: Measurement of biofilm inhibition or disruption by probiotics.
• Immunomodulatory Assays: Analysis of probiotic effects on host immune responses.
• Anti-Invasion Assays: Determining if probiotics can prevent pathogens from invading host cells.
• Co-aggregation Assays: Assessing the ability of probiotics to aggregate with pathogens, potentially hindering their ability to colonize host surfaces.

In Vivo Efficacy Studies (Animal Models)

• Animal Model Infection Studies: For promising probiotic candidates identified in vitro, we conduct in vivo efficacy studies using relevant animal models of infection. These studies assess the ability of probiotics to prevent or treat infections, reduce pathogen load, and improve host health.
• Impact on Host Immune Response: Assessing the probiotic's ability to modulate the immune system to fight infection.

Data Analysis and Reporting

Our team of expert scientists and bioinformaticians performs rigorous data analysis using appropriate statistical methods. We provide comprehensive reports summarizing the study findings, including detailed methodologies, results, and interpretations.

Deliverables

Turnaround Time

The typical timeframe for this service ranges from 8 to 16 weeks, depending on the complexity of the study design, the number of probiotic strains and pathogens evaluated, and the specific in vivo models used.