We selectively recover D. welbionis from fecal material or strain repositories under rigorously controlled anoxia (O2 < 1 ppm). Primary screens quantify growth kinetics, redox tolerance, and SCFA output—with emphasis on butyrate—so you can rank D. welbionis isolates by fitness and functional readouts before deeper characterization and banking.
To lock in taxonomic certainty, we combine 16S rRNA sequencing with whole-genome sequencing to confirm D. welbionis identity, resolve related Oscillospiraceae neighbors, and annotate biosynthetic potential. We deliver version-controlled, traceable strain dossiers so every experiment referencing D. welbionis remains auditable across studies, sites, and time points.
We map carbohydrate utilization of D. welbionis across inulin, resistant starch classes, and other dietary fibers, quantifying SCFA stoichiometry (especially butyrate), lactate cross-feeding, and gas profiles. These datasets drive media optimization and synbiotic pairing strategies centered on D. welbionis productivity and stability in realistic substrate regimes.
Using standardized in-vitro platforms, we profile the metabolome (SCFAs, bioactive lipids) and quantify readouts related to barrier-associated markers and inflammatory signals to outline potential modes of action for D. welbionis. Results include dose-response curves for cells, cell-free supernatants, and fractionated metabolites—enabling mechanism-driven design without clinical interpretation.
We deploy intestinal epithelial monolayers, gut–liver interfaces, and immune co-cultures (e.g., Caco-2/HT29-MTX, primary or iPSC-derived cells) to quantify adhesion, TEER, tight-junction markers, and pathway reporters in response to D. welbionis biomass or conditioned media under anaerobe-aware handling. Outputs benchmark D. welbionis against reference commensals.
Macrophage and dendritic-cell assays track cytokines, chemokines, and surface phenotypes after exposure to D. welbionis cells or supernatants. We integrate endotoxin controls, heat-killed comparators, and fractionation to separate pattern-recognition effects from SCFA-driven responses, defining a safe and informative operating window for D. welbionis investigations.
Our anaerobic bioprocessing team develops and scales robust fermentations for D. welbionis—controlling pH, redox potential, substrate feeding, and agitation to stabilize biomass and butyrate output. We harmonize upstream parameters with downstream needs (concentration, washing, cryo-protectants) to support repeatable in-vitro studies and pre-clinical sample logistics.
Recognizing oxygen sensitivity, we design freezes-dried, microencapsulated, or oxygen-buffered formulations that protect D. welbionis during storage and simulated GI transit. We tune excipients and capsule matrices to maximize viable recovery, compatibility with carriers, and shelf stability—aligned to shipping, dosing, and study-specific handling constraints.
