Engineering Bacillus subtilis Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
Bacillus subtilis is a symbiotic bacterium, found primarily in soil, capable of forming metabolically inactive dehydrated endospores that allow survival in nutrient-free environments. Due to its ideal genetic structure, it is used in molecular microbiology research. It has been thoroughly studied for wide use as a microbial cell factory in industrial production. Bacillus subtilis grows rapidly, has clear genetic background, GRAS physiological advantages, is an ideal transgenic engineering bacteria, and has a strong development significance. As a probiotic, it has a good effect on regulating intestinal flora, improving intestinal immunity, inhibiting harmful microorganisms, secreting bioactive substances, etc. It is often used as a feed growth supplement and an effective alternative to antibiotics. Vegetative and spore forms of Bacillus subtilis are also used as vaccine carriers to deliver antigens of infectious pathogens.
Clustered regularly interspaced short palindromic repeats has revolutionized the way we design genomes. Its simplicity and modularity have allowed the development of a large number of tools for editing genomes and controlling gene expression. Many tools have been successfully developed, including double-stranded and single-stranded cutting gene editing tools for point mutation, gene insertion, etc., to build toolkits for such Gram-positive models and important industrial chassis. Application of the genome editing and transcriptional regulation makes strain modification much more convenient. Bacillus subtilis is more suitable as a chassis cell because of its non-pathogenicity and good protein secretion ability. The rod-shaped Gram-positive bacteria Bacillus subtilis is well characterized and easily amendable to genetic engineering. Bacillus subtilis has inherent MEP pathway genes. It is widely used in the industrial production of proteins and has aroused people's interest.
Engineering Bacillus subtilis Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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PAGE
or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain.
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Target gene name.
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Target gene sequence. (If the whole genome sequence is unavailable.)
How Bacillus subtilis Strain Construction Service Can Assist Your Project
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Construct the target strains according to the needs of clients or referring to the published scheme.
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Optimize the program to improve the success rate.
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Delivery of the constructed target strains (knockout or integrated).
Advantages
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Lack of pathogenicity and endotoxin.
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No pathogenicity, good environmental compatibility.
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Unlikely to generate resistance
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Being able to secrete a variety of enzymes and antibiotics.
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Genetic engineering adaptability.
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Widely used in medicine, agriculture, and industry.
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Genetic manipulation is relatively easy.
Frequently Asked Questions
What are the gene editing technologies applicable to Bacillus subtilis?
There are several gene editing technologies applicable to
Bacillus subtilis:
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Genome editing system: It allows for targeted gene knockouts, insertions, and modifications. For multiplex genome editing, enabling simultaneous modifications of multiple genes.
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Homologous Recombination (HR): Traditional method relying on the natural ability of Bacillus subtilis to incorporate exogenous DNA through homologous recombination.
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Transposon Mutagenesis: Utilizes transposable elements to create random mutations, useful for identifying gene functions.
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Site-Directed Mutagenesis: Techniques like PCR-based methods to introduce specific mutations at particular sites in the genome.
Each of these technologies has its advantages and applications, depending on the specific goals of the project.
What are the applications of Bacillus subtilis in the field of probiotics?
Bacillus subtilis shines in the world of probiotics. Here are some highlights:
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Gut Health: Bacillus subtilis supports gastrointestinal health by promoting a balanced gut microbiota.
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Digestive Aid: It helps alleviate digestive issues like IBS and improves overall digestive function.
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Immune Boost: This bacterium enhances the immune response, helping fend off infections.
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Antimicrobial Power: Its natural antimicrobial properties can prevent and treat infections.
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Nutrient Absorption: B. subtilis aids in breaking down food, improving nutrient absorption.
Published Data
Isolation of Potential Probiotic Bacillus spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia2, 3
Abstract
This study aimed to search for potential probiotic Bacillus from the gut of Nile tilapia and construct recombinant probiotics to enhance the immune response of Nile tilapia. According to the screening results, the recombinant probiotics of CC chemokine protein (pBESOn-CC) were constructed by selecting Bacillus B29 which was closely related to Bacillus subtilis. Both wild-type and recombination-type probiotics significantly improved the phagocytic activity of Nile tilapia, indicating that probiotics can stimulate the immune system of Nile tilapia through different processes. Interestingly, dietary supplementation of recombinant probiotics had a stronger immune response enhancement effect than wild-type strains.
Fig.2 Effect of dietary probiotic supplementation on phagocytic activity and phagocytic index of phagocytic cells in PBLs.2, 3
Resources
Creative Biolabs has extensive project experience in the field of probiotic research, and if you are working with Bacillus subtilis, feel free to contact us to discuss your project, including recombinant strain construction.
References
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Liao, Chaoyong, et al. "Advances in enhanced menaquinone-7 production from Bacillus subtilis." Frontiers in Bioengineering and Biotechnology 9 (2021): 695526.
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Nakharuthai, Chatsirin, et al. "Isolation of Potential Probiotic Bacillus spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia." Animals 13.6 (2023): 986.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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