Engineering Lactobacillus reuteri Services for Live Biotherapeutics Drug Discovery
Background Services Advantages FAQs Published Data Resources
Background
The efficient editing of the genome of strains of industrial and medical importance is crucial. This allows scientists to improve microbial strains promptly and will enhance their understanding of how health-promoting plays their role. Efficient genome editing will promote the understanding of the physiological characteristics of probiotics and their molecular mechanisms of interaction with hosts and host microbiota, thereby further promoting the development of next-generation design probiotics with robustness and customized functionality. With the development of gene editing-based genome editing strategies and other synthetic biology technologies, the research and application of these probiotics in the food and pharmaceutical industries will be further enhanced. In animal disease models, Lactobacillus reuteri (L. reuteri) has been shown to function as a therapeutic delivery platform. The combination of gene editing and recombinant enzyme T (RecT) mediated single-stranded DNA (ssDNA) oligonucleotide recombination in L. reuteri provides an efficient editing cell selection for oligonucleotide mediated chromosomal deletions within the 1 kb region. The gene editing system has been used for the first time in reverse selection to assist ssDNA-mediated recombination in L. reuteri. gene editing-assisted ssDNA recombination has been used in the production of bacteriophages in L. reuteri.
Fig.1 Genetic tool availability and beneficial probiotic properties of L. reuteri.1
Engineering Lactobacillus reuteri Services
Services
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Gene knock-out
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Gene knock-in/Gene insertion
Deliverables
Engineered strain in glycerol stock
Quality Control
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Sequencing Validation
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Western Blot Validation (Optional)
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PAGE
or cell-based assay of your interest.
Delivery Time
Starting from 4-6 weeks.
Materials from Clients
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Overall construction scheme.
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Host strain
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Target gene name
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Target gene sequence, if the whole genome sequence is unavailable
How L. reuteri Strain Construction Service Can Assist Your Project
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Construct the target strains according to the needs of clients or referring to the published scheme.
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Optimize the program to improve the success rate.
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Delivery of the constructed target strains (knockout or integrated).
Advantages
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The availability of high-throughput genome editing tools.
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L. reuteri's ability to thrive in the gut ecosystem.
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L. reuteri has an extraordinarily low mutation rate, which is expected to improve the genetic stability of the recombinant organism.
Frequently Asked Questions
What are the genome editing tools available in L. reuteri?
ssDNA recombineering and gene editing. The advantages of ssDNA recombination are that it is site-specific, not highly mutagenic, allows for efficient subtle genomic modifications, and the selection of mutants without selection for antibiotic markers. gene editing has the advantages of enabling programmable, precise genome editing, high efficiency for small deletions, marker-free selection, and multiplexing potential in which multiple deletions or genome modifications can be made simultaneously.
What are the functions of engineered probiotics (EPs)?
A. EPs can be used to treat/manage chronic and congenital diseases. They can help reduce symptoms of hyperammonemia by helping the intestine process excess ammonia and convert it into a non-toxic form. Similarly, EPs can be used to break down Phe, providing an alternative treatment option for phenylketonuria (PKU). Bacterial phenylalanine ammonia-lyase (PAL) has a potential therapeutic effect on PKU when administered orally by
Escherichia coli Nissle 1917 (EcN) and
L.
reuteri.
Published Data
Secretion of recombinant interleukin-22 by engineered L. reuteri reduces fatty liver disease in a mouse model of diet-induced obesity.
Abstract
Using a mouse model of diet-induced obesity, the scientists evaluated various markers of metabolic syndrome after treatment with L. reuteri and recombinant derivatives. They reported that the probiotic L. reuteri reduced fatty liver in diet-induced obesity mouse models. The expression of anti-inflammatory cytokine interleukin-22 by engineered L. reuteri can promote the expression of a renewable islet-derived 3γ antibacterial C-type lectin in the intestine, thereby alleviating alcoholic liver disease.
Fig.2 L. reuteri VPL3461 (LR-IL-22 group) improves diet-induced fatty liver disease.1
Resources
Engineered probiotics could be an effective and innovative alternative therapy for the treatment and prevention of human disease. Creative Biolabs has accumulated a wealth of experience in the field of probiotic research. We have established an advanced gene editing technology platform and launched the L. reuteri genome editing service. If you are engaged in this research, you are welcome to contact us at any time to communicate and discuss our services or technologies.
Reference
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Oh, Jee-Hwan, et al. "Secretion of recombinant interleukin-22 by engineered Lactobacillus reuteri reduces fatty liver disease in a mouse model of diet-induced obesity." MSphere 5.3 (2020): 10-javascript;1128.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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