We isolate B. vulgatus from validated sources under strict anaerobic conditions. Functional screening evaluates adhesion to epithelial cells, immune modulation, and pathogen exclusion, allowing us to prioritize strains with the strongest biological potential for downstream research and development applications.
Accurate identification employs 16S rRNA sequencing and whole-genome sequencing to resolve strain-level differences. Our analysis clarifies Bacteroides–Phocaeicola transitions, with deliverables including SNP typing, polysaccharide utilization loci (PUL) annotation, and curated genomic packages to support registration, traceability, and consistent data interpretation.
We establish optimized fermentation strategies under controlled anaerobic conditions. Parameters such as pH, osmolality, and nutrient flux are finely tuned to achieve stable biomass and reproducible metabolite yields. This approach minimizes product inhibition and ensures reliable outcomes during scale-up development.
Our downstream workflows combine centrifugation, filtration, and concentration methods to maximize recovery of viable cells. For metabolite-centered projects, supernatants enriched with SCFAs undergo validated analytical testing, ensuring consistent, contamination-free outputs that support advanced microbiome mechanism studies and product design.
Formulation experts optimize lyophilization and cryopreservation strategies with protective excipients such as trehalose or skim milk. We perform accelerated and real-time stability studies, using CFU counts and flow cytometry to guarantee viability and integrity of B. vulgatus across long storage periods.
We assess B. vulgatus activity using epithelial and immune co-cultures, measuring TEER, cytokine secretion, and competitive exclusion. These readouts are integrated with SCFA profiling and PUL expression analysis, providing mechanistic insights into host–microbe interactions and strain-specific functional benefits.
Our platforms include organoids and Transwell models maintained under physiologic oxygen. These advanced systems quantify epithelial barrier integrity, immune signaling, and microbial adhesion, generating reproducible, human-relevant data on B. vulgatus interactions and supporting in-depth microbiome and host biology studies.
Defined carbohydrate panels—such as inulin, arabinoxylan, and xylooligosaccharides—are used to assess substrate utilization and SCFA production by B. vulgatus. We map outputs to PUL activity, generating detailed data that inform synbiotic design and personalized nutritional intervention research strategies.
Fig.1 B. vulgatus strain preparation.
