Using strict anaerobic workflows and selective media, we isolate A. hadrus from fecal sources or synthetic communities, then tier candidate clones by butyrate yield, lactate/acetate utilization, and growth kinetics. Screening funnels are designed to surface high-performing A. hadrus strains with clear links between phenotype, media composition, and scalability.
We confirm A. hadrus identity and purity via 16S rRNA, WGS, and MALDI-TOF, excluding near-neighbor taxa. Reports include phylogeny and functional annotations of butyrate pathways (e.g., lactate-to-butyrate conversion), enabling rapid go/no-go decisions on A. hadrus candidates and traceable audit trails for downstream scale-up.
We design A. hadrus species/strain-specific qPCR/ddPCR primers and probes for real-time monitoring in fermenters, in vitro co-cultures, and animal matrices. Assays emphasize limit-of-detection, dynamic range, and matrix tolerance, ensuring sensitive, specific quantification of A. hadrus across development stages and sample types.
We optimize A. hadrus in controlled, oxygen-managed bioreactors, tuning pH, carbon sources (including lactate/acetate co-feeding), feed strategies, and agitation profiles. Data packages capture growth curves, acidification kinetics, SCFA trajectories, and CPP/CPK trends to evaluate scale-up feasibility for A. hadrus while preserving target phenotypes.
We deliver A. hadrus as lyophilized powders or glycerol stocks from milliliter to multi-liter campaigns, with batch records, preliminary stability insights, and storage/transport guidance. Tech memos summarize critical parameters and scale-up suggestions so your team can confidently plan the next run for A. hadrus.
We map A. hadrus carbon-use phenotypes across resistant-starch hydrolysates, oligosaccharides, and lactate/acetate co-substrates, resolving SCFA spectra (butyrate-focused) and flux preferences. These profiles connect medium composition to functional outputs, supporting formulation hypotheses and strain-substrate matching for A. hadrus.
We build MoA-aligned assays for A. hadrus to quantify butyrate generation, lactate clearance, bile acid co-metabolism, and metabolite signaling axes. Multi-omics (targeted metabolomics, transcriptomics) provides mechanistic readouts to validate A. hadrus hypotheses and prioritize leads with consistent functional signatures.
In epithelial/mucus models and gut organoids, we evaluate A. hadrus effects on barrier function (TEER), mucin expression, inflammatory markers, and metabolite-mediated responses. These studies contextualize A. hadrus outputs within host biology, creating a coherent evidence chain for downstream decisions.
