We isolate and screen L. rhamnosus from diverse sources (e.g., fermented foods, human gut–associated samples) using trait-led selection. Panels can include acid/bile tolerance, growth kinetics, antimicrobial activity, and preliminary adhesion proxies—so only L. rhamnosus candidates with measurable, project-relevant performance advance.
For programs requiring advanced functional prototypes, we design recombinant L. rhamnosus constructs using contemporary genetic toolkits to express bioactive payloads, enzymes, or display modules on the cell surface. Each L. rhamnosus design is paired with verification steps (genotype/phenotype) to keep engineering outcomes interpretable and reproducible.
We confirm L. rhamnosus identity with polyphasic workflows: 16S rRNA sequencing plus higher-resolution genomic strategies such as WGS and comparative mapping. This approach safeguards against misassignment within closely related taxa and supports traceable strain records—especially critical when L. rhamnosus performance is highly strain dependent.
We evaluate core probiotic-relevant functions of L. rhamnosus using study-aligned assays (barrier integrity, pathogen inhibition models, adhesion/colonization proxies, and metabolite-linked readouts). The goal is to connect L. rhamnosus strain behavior to an evidence-backed, testable mechanism-of-action narrative that supports rational selection and optimization.
We profile immunology-facing signals triggered by L. rhamnosus using controlled in vitro immune cell interaction models. Typical outputs include cytokine patterns (e.g., IL-10, TNF-α) and immune-cell activation markers—positioning each L. rhamnosus strain on a comparative immunomodulation map rather than a single endpoint.
We optimize upstream conditions for L. rhamnosus to achieve consistent biomass and reproducible quality attributes. Fermentation development can include media optimization, pH/DO control strategies, harvest timing, and scale-up parameters—so your L. rhamnosus strain performs predictably from bench to larger batches.
We develop stability-driven formulations for L. rhamnosus (e.g., powders or encapsulated formats) using appropriate cryoprotectants and microencapsulation approaches to protect viability during processing and storage. Each L. rhamnosus formulation strategy is guided by measured survival curves, not assumptions.
We perform rigorous stability testing for L. rhamnosus across practical stressors: temperature/humidity, accelerated conditions, and exposure to simulated gastric/intestinal environments. Outputs include viability retention, functional persistence (where applicable), and shelf-life projections to support development decisions and tech transfer.
