Lactobacillus rhamnosus is a short Gram-positive homofermentative facultative anaerobic non-spore-forming rod that often appears in chains.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Lactobacillus rhamnosus is a short Gram-positive homofermentative facultative anaerobic non-spore-forming rod that often appears in chains.
Target
Lactobacillus
Genus
Lactobacillus
Strain Designation
899-1
Application
Study and research
Type Strain
No
Culture Medium
MRS
Culture Conditions
37°C; Facultative anaerobic
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel.
Alternative Names
Lactobacillus rhamnosus; 899-1
FAQs Customer Reviews Related Products
What are the initial steps for culturing Lactobacillus rhamnosus from a lyophilized state?
To culture Lactobacillus rhamnosus, rehydrate the lyophilized powder in sterile MRS broth. Incubate under anaerobic conditions at 37°C for 24-48 hours to achieve optimal bacterial growth before proceeding with experimental applications.
What are the considerations for scaling up the culture of Lactobacillus rhamnosus?
When scaling up, ensure the use of a bioreactor with controlled pH, temperature, and oxygen levels. Gradual adaptation to larger volumes prevents stress and maintains the viability and metabolic activity of the bacteria.
How should Lactobacillus rhamnosus be prepared for inclusion in a probiotic formulation?
To include in a probiotic formulation, concentrate the bacteria through centrifugation, resuspend in a cryoprotectant solution, and lyophilize. This process ensures high viability and stability of Lactobacillus rhamnosus in the final product.
What methods are used to quantify the bacterial concentration of Lactobacillus rhamnosus in a sample?
Quantification can be performed using colony-forming unit (CFU) counts on MRS agar plates, qPCR for specific genetic markers, or spectrophotometric measurements correlated with bacterial density standards.
What are the recommended steps for cryopreserving Lactobacillus rhamnosus?
For cryopreservation, mix the bacterial culture with a cryoprotectant such as glycerol or DMSO. Aliquot into cryovials and store at -80°C. This procedure maintains high cell viability for long-term storage.
Outstanding Stability for Probiotic Research
Creative Biolabs' Lactobacillus rhamnosus 899-1 demonstrates outstanding stability, making it ideal for probiotic research. Its high viability ensures consistent and reliable experimental results.
Excellent Performance in Gut Microbiota Studies
The Lactobacillus rhamnosus 899-1 from Creative Biolabs performs excellently in gut microbiota studies. Its robustness and high activity significantly enhance the quality of research outcomes.
Reliable for Functional Food Development
Creative Biolabs offers a reliable Lactobacillus rhamnosus 899-1 product, perfect for developing functional foods. Its consistent quality supports advanced research and product innovation.
Superior Quality for Immunomodulatory Research
The Lactobacillus rhamnosus 899-1 from Creative Biolabs is of superior quality, particularly beneficial for immunomodulatory research. It provides reliable data for studies on immune response.
Ideal for Anti-inflammatory Studies
This product is ideal for anti-inflammatory studies. Its high efficacy and stability ensure accurate results, making it a top choice for researchers in this field.
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