Creative Biolabs isolates and enriches C. beijerinckii from soil, fecal, feed, and fermentation samples using selective anaerobic culturing and spore-based recovery strategies. Candidate C. beijerinckii strains are screened for solvent yields, sugar utilization patterns, growth kinetics, and tolerance to lignocellulosic hydrolysate inhibitors. This systematic isolation and screening pipeline builds robust C. beijerinckii strain collections aligned with your downstream bioprocess or microbiome objectives.
To avoid mis-assignment among closely related solventogenic clostridia, C. beijerinckii isolates are confirmed at Creative Biolabs by a combination of 16S rRNA sequencing, whole-genome or targeted gene panels, fatty acid profiles, and phenotypic tests. Our polyphasic identification workflow verifies that cultures are authentic C. beijerinckii, free from co-cultured Clostridium species or facultative anaerobes, and suitable for use as reference strains, production strains, or microbiome research tools.
Creative Biolabs performs strictly controlled anaerobic fermentations with C. beijerinckii to characterize and optimize ABE production. We design experiments around pH control, redox balance, and nutrient regimes to map acidogenic and solventogenic phases, quantify acetone, butanol, and ethanol ratios, and evaluate performance on pure sugars and lignocellulosic hydrolysates. Our bioreactor and serum-bottle platforms support rapid iteration of C. beijerinckii fermentation strategies.
For teams needing consistent material, Creative Biolabs scales C. beijerinckii from bench cultures into lab-scale bioreactors to generate biomass, spores, culture supernatants, and solvent-rich broths. Process parameters are tuned so that the resulting batches recapitulate industrially relevant ABE titers, solvent ratios, and by-product profiles. This lab-scale production capability supplies material for formulation development, omics analyses, and microbiome or co-culture test systems.
Beyond fermentation, Creative Biolabs designs downstream workflows tailored to C. beijerinckii–based processes. Our teams evaluate cell separation methods, solvent stripping or pervaporation options, in situ product recovery strategies, and fractionation of aqueous and organic phases. We generate process concepts that help customers move from laboratory C. beijerinckii runs to robust development-stage flowsheets, with data on yield, purity, and energy demand for each step.
Solvent toxicity, pH shifts, and lignocellulosic inhibitors can severely impact C. beijerinckii performance. Creative Biolabs characterizes stress responses by exposing C. beijerinckii to gradients of butanol, acids, phenolic compounds, oxygen traces, and osmotic pressure, then tracking growth, solvent production, and viability. We combine physiological data with transcriptomic or targeted gene expression analyses to reveal stress-response mechanisms and guide strain engineering or process control strategies.
To ensure that C. beijerinckii retains its solventogenic phenotype and viability during storage and shipment, Creative Biolabs develops stabilization strategies including cryopreservation, lyophilization, and spore-based formats. We assess recovery, growth kinetics, solvent profiles, and sporulation behavior after storage under different conditions. These stabilization protocols support use of C. beijerinckii as a reference strain, production starter, or component of microbiome-related research products.
Building on our live biotherapeutic platform, Creative Biolabs engineers C. beijerinckii as a research chassis for microbiome-focused applications. We design metabolic rewiring for targeted solvent or short-chain fatty acid production, introduce genetic circuits for enhanced stress tolerance, and evaluate compatibility with encapsulation or co-formulation systems. These engineered C. beijerinckii strains support gut model studies and postbiotic development without positioning them as finished consumer probiotics.
