We enrich spores and vegetative cells under strict anaerobiosis, isolate colonies on selective media, and deploy high-throughput pre-screens (butyrate production, gas profiles, bile/acid tolerance). Parallel phenotyping identifies robust, fast-growing isolates with desirable spore yields and functional readouts suited to your downstream models.
Strain identity and safety markers are resolved with 16S rRNA sequencing plus whole-genome sequencing (WGS). We annotate carbohydrate-active enzymes (CAZymes), predict metabolic routes to butyrate, assess plasmids, and screen for known virulence or AMR determinants to support risk assessment in research contexts.
Because C. butyricum is a spore former, we quantify heat-resistant spore counts, engineer stress-protection matrices (e.g., trehalose, skim milk, proteins), and test survival across acid, bile, moisture, oxygen, and temperature excursions. Stability reports include accelerated and real-time studies aligned to your storage conditions and logistics.
We formulate spores or mixed populations for oral or experimental delivery, evaluating microencapsulation (alginate, HPMC), granulation, and excipient compatibility. GI-simulation assays track release kinetics and viability; we also examine co-formulation with carbohydrates that favor butyrate flux from lactate/acetate where relevant.
We map carbon utilization and flux to SCFAs (GC/LC-MS for butyrate, acetate, lactate). Multi-substrate panels and transcript-level insights clarify how CAZyme repertoires drive fermentation across fiber classes—data you can use to tune media, diet components, or synbiotic designs.
Under CLSI-aligned anaerobic conditions, we determine MICs for antibiotics of interest and evaluate probiotic–antibiotic co-exposure viability. These studies quantify compatibility and resilience—important for model design when antibiotics are part of your experimental system.
From bench to pilot, we optimize media, pH and redox control, gas transfer, and fed-batch regimes to maximize spore yield and metabolic outputs. Harvest, washing, drying (including lyophilization), and in-process QC are integrated to deliver scalable, reproducible lots ready for extended research studies.
Creative Biolabs develops engineered C. butyricum for research, using CRISPR editing and pathway modulation to boost butyrate output, substrate range, and spore resilience. Modular promoters and markerless constructs improve stability. Deliverables include annotated genomes, benchmarks, and scale-up guidance for consortia and formulations.
