Clostridium butyricum is a strictly anaerobic endospore-forming Gram-positive butyric acid-producing bacillus.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-061FG
Product Information
Product Overview
Clostridium butyricum is a strictly anaerobic endospore-forming Gram-positive butyric acid-producing bacillus.
Target
Clostridium
Genus
Clostridium
Application
Study and research
Type Strain
Yes
Culture Medium
Blood agar
Culture Conditions
37°C; Anaerobic
Characteristics
G+C mol% : 28.0. Gram-positive, cells are rod-shaped, slightly curved and arranged singly. Bud elliptic, middle to secondary end, cysts do not dilate.
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Clostridium is a genus of rod-shaped, usually Gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. The majority of species are obligate anaerobes. As the predominant bacteria in gut, Clostridium species exert lots of benefits to body health via interacting with intestine directly or indirectly. Clostridium species are potent candidates to alleviate dysfunctions and disorders in intestine, they have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In view of their salutary performances, Clostridium species have a huge potential as probiotics.
Alternative Names
Clostridium butyricum; 19398
FAQs Customer Reviews Related Products
What are the optimal growth conditions for Clostridium butyricum?
Clostridium butyricum thrives in strictly anaerobic conditions. It is recommended to use pre-reduced media such as Reinforced Clostridial Medium (RCM) and maintain an incubation temperature of 37°C. Anaerobic conditions can be achieved using an anaerobic chamber or gas pack systems.
What steps should be followed for initial culturing of Clostridium butyricum?
When handling Clostridium butyricum, it is advised to first establish growth in a broth medium for 24 hours before transferring to solid media. This bacterium is typically cultured on trypticase soy agar with defibrinated sheep blood under anaerobic conditions. Ensure the use of freshly prepared, pre-reduced media to prevent exposure to oxygen.
How should Clostridium butyricum be stored and revived from frozen stock?
Clostridium butyricum should be stored at -80°C in a cryoprotective agent such as glycerol. To revive the culture, thaw the vial in an anaerobic environment, then aseptically transfer the contents to a suitable pre-reduced liquid medium. It is critical to maintain anaerobic conditions throughout the process to ensure cell viability and optimal growth.
Can Clostridium butyricum be used in applications beyond basic research?
Clostridium butyricum is intended strictly for laboratory research use and is not approved for clinical, diagnostic, or therapeutic applications in humans or animals. Its primary use includes studying metabolic processes, fermentation pathways, and potential probiotic effects under controlled laboratory conditions.
How is the purity and identity of Clostridium butyricum verified?
The purity and identity of Clostridium butyricum are verified through whole-genome sequencing and phenotypic assays. For quality control, colony morphology can be assessed on Brucella Blood Agar Plates. Any changes in growth medium or handling procedures should be carefully documented, as deviations can affect the recovery and functionality of the culture.
Exceptional Growth Consistency
The Clostridium butyricum; 19398 from Creative Biolabs showed exceptional growth consistency in anaerobic conditions. It was pivotal in our fermentation process studies, delivering reliable and reproducible results.
Reliable Anaerobic Culture Clostridium butyricum; 19398 provided excellent results in anaerobic culture conditions. Its robust growth and stability made it a key component in our metabolic pathway research.
Superior Performance in Probiotic Studies
Utilizing Clostridium butyricum; 19398 in our probiotic efficacy studies yielded superior results. The strain's stability and high viability under anaerobic conditions facilitated thorough and accurate research outcomes.
Ideal for Biochemical Pathway Analysis Clostridium butyricum; 19398 from Creative Biolabs proved to be ideal for analyzing biochemical pathways. Its consistent growth and metabolic activity provided clear insights into butyric acid production.
High-Quality Strain for Fermentation Research
The high-quality Clostridium butyricum; 19398 from Creative Biolabs was instrumental in our fermentation research. Its predictable growth patterns and resilience in anaerobic environments ensured accurate and dependable data collection.
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