Clostridium difficile is a Gram-positive species of spore-forming bacteria. It is is catalase- and superoxide dismutase-negative.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-065FG
Product Information
Product Overview
Clostridium difficile is a Gram-positive species of spore-forming bacteria. It is is catalase- and superoxide dismutase-negative.
Target
Clostridium
Genus
Clostridium
Application
Study and research
Type Strain
No
Culture Medium
Thioglycolate medium
Culture Conditions
37°C; Anaerobic
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Clostridium is a genus of rod-shaped, usually Gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. The majority of species are obligate anaerobes. As the predominant bacteria in gut, Clostridium species exert lots of benefits to body health via interacting with intestine directly or indirectly. Clostridium species are potent candidates to alleviate dysfunctions and disorders in intestine, they have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In view of their salutary performances, Clostridium species have a huge potential as probiotics.
Alternative Names
Clostridium difficile
FAQsPublished Data Customer Reviews Related Products
How should Clostridium difficile be stored for long-term preservation?
For long-term preservation, Clostridium difficile should be stored at -80°C. Cryoprotectants such as glycerol can be added to the storage medium to enhance the viability of the bacterial cells during freezing. Proper labeling and documentation are essential to maintain sample integrity.
What are the key diagnostic methods for detecting Clostridium difficile in stool samples?
Clostridium difficile is primarily used in research focused on gastrointestinal diseases, antibiotic-associated diarrhea, and microbiome studies. Its role in toxin production and pathogenicity makes it a valuable model for studying infection mechanisms and testing therapeutic interventions.
How can the virulence of Clostridium difficile strains be assessed?
The virulence of Clostridium difficile strains can be assessed by evaluating toxin production, spore formation, and adherence to intestinal cells. Assays such as cell cytotoxicity neutralization and animal models are commonly used to study the pathogenic potential of different strains.
How can Clostridium difficile toxin production be assessed in the laboratory?
Toxin production can be assessed using cell cytotoxicity assays, enzyme-linked immunosorbent assays (ELISA), and quantitative PCR for toxin gene expression. These methods help determine the virulence potential of different strains.
What precautions should be taken to prevent the spread of Clostridium difficile in the laboratory?
To prevent the spread, always use dedicated equipment, perform regular decontamination of surfaces with bleach, and ensure proper waste disposal. Implementing strict hand hygiene protocols and using PPE are also critical steps.
Daily average diarrhea scores (A) and the number of C. difficile in feces (B) of rats orally infected with C. difficile VPI10463
The study explores the development of a novel rat model for Clostridium difficile infection (CDI). Traditional hamster models for CDI, often used for analysis, present significant differences from human CDI, notably high mortality without exhibiting diarrhea. This study attempts to create a more accurate rat model that mimics human CDI symptoms using endogenous C. difficile, metronidazole, and egg white. The study demonstrates that Syrian Golden hamsters infected with hypertoxigenic and low toxigenic strains of C. difficile exhibit high mortality rates (up to 100%) without significant diarrhea. In contrast, Wistar rats pretreated with metronidazole and given egg white developed non-lethal diarrhea, more closely resembling human CDI. Probiotic treatment with Clostridium butyricum MIYAIRI 588 significantly reduced the incidence and severity of diarrhea in rats. This model offers a promising approach for studying CDI mechanisms and evaluating probiotic treatments, indicating that C. butyricum could mitigate CDI risks by decreasing diarrhea and altering intestinal microbiota dynamics.
Oka, Kentaro, et al. "Establishment of an endogenous Clostridium difficile rat infection model and evaluation of the effects of Clostridium butyricum MIYAIRI 588 probiotic strain." Frontiers in Microbiology 9 (2018): 1264. Distributed under Open Access license CC BY 4.0, without modification.
Superior Quality for Microbiome Research
The Clostridium difficile strain from Creative Biolabs has significantly enhanced our microbiome studies. Its reliability and consistency are unparalleled, providing excellent results in our gut flora research.
Exceptional Performance in Toxin Production Studies
Using Creative Biolabs' Clostridium difficile, our toxin production assays have shown remarkable accuracy and repeatability. This strain is indispensable for studying pathogenicity and toxin-related mechanisms.
Highly Reliable for Antibiotic Resistance Research
Creative Biolabs' Clostridium difficile strain has proven extremely reliable in our antibiotic resistance studies. Its stability and high-quality make it a top choice for detailed research.
Excellent for Gastrointestinal Infection Models
This strain has provided outstanding results in our gastrointestinal infection models, offering deep insights into infection dynamics and host responses. Highly recommended for advanced research applications.
Ideal for Toxin Gene Expression Studies
Our studies on toxin gene expression have greatly benefited from the use of Creative Biolabs' Clostridium difficile strain. Its robust performance and accuracy are unmatched.
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