Clostridium difficile is a Gram-positive species of spore-forming bacteria. It is is catalase- and superoxide dismutase-negative.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT | Size | Price | Quantity | |
---|---|---|---|---|
LBST-065FG |
|
Product Information | |
---|---|
Product Overview | Clostridium difficile is a Gram-positive species of spore-forming bacteria. It is is catalase- and superoxide dismutase-negative. |
Target | Clostridium |
Genus | Clostridium |
Application | Study and research |
Type Strain | No |
Culture Medium | Thioglycolate medium |
Culture Conditions | 37°C; Anaerobic |
Risk Group | 2 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
---|---|
Introduction | Clostridium is a genus of rod-shaped, usually Gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. The majority of species are obligate anaerobes. As the predominant bacteria in gut, Clostridium species exert lots of benefits to body health via interacting with intestine directly or indirectly. Clostridium species are potent candidates to alleviate dysfunctions and disorders in intestine, they have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In view of their salutary performances, Clostridium species have a huge potential as probiotics. |
Alternative Names | Clostridium difficile |
For long-term preservation, Clostridium difficile should be stored at -80°C. Cryoprotectants such as glycerol can be added to the storage medium to enhance the viability of the bacterial cells during freezing. Proper labeling and documentation are essential to maintain sample integrity.
Clostridium difficile is primarily used in research focused on gastrointestinal diseases, antibiotic-associated diarrhea, and microbiome studies. Its role in toxin production and pathogenicity makes it a valuable model for studying infection mechanisms and testing therapeutic interventions.
The virulence of Clostridium difficile strains can be assessed by evaluating toxin production, spore formation, and adherence to intestinal cells. Assays such as cell cytotoxicity neutralization and animal models are commonly used to study the pathogenic potential of different strains.
Toxin production can be assessed using cell cytotoxicity assays, enzyme-linked immunosorbent assays (ELISA), and quantitative PCR for toxin gene expression. These methods help determine the virulence potential of different strains.
To prevent the spread, always use dedicated equipment, perform regular decontamination of surfaces with bleach, and ensure proper waste disposal. Implementing strict hand hygiene protocols and using PPE are also critical steps.
Daily average diarrhea scores (A) and the number of C. difficile in feces (B) of rats orally infected with C. difficile VPI10463
The study explores the development of a novel rat model for Clostridium difficile infection (CDI). Traditional hamster models for CDI, often used for analysis, present significant differences from human CDI, notably high mortality without exhibiting diarrhea. This study attempts to create a more accurate rat model that mimics human CDI symptoms using endogenous C. difficile, metronidazole, and egg white.
The study demonstrates that Syrian Golden hamsters infected with hypertoxigenic and low toxigenic strains of C. difficile exhibit high mortality rates (up to 100%) without significant diarrhea. In contrast, Wistar rats pretreated with metronidazole and given egg white developed non-lethal diarrhea, more closely resembling human CDI. Probiotic treatment with Clostridium butyricum MIYAIRI 588 significantly reduced the incidence and severity of diarrhea in rats. This model offers a promising approach for studying CDI mechanisms and evaluating probiotic treatments, indicating that C. butyricum could mitigate CDI risks by decreasing diarrhea and altering intestinal microbiota dynamics.
Oka, Kentaro, et al. "Establishment of an endogenous Clostridium difficile rat infection model and evaluation of the effects of Clostridium butyricum MIYAIRI 588 probiotic strain." Frontiers in Microbiology 9 (2018): 1264.
Click the button below to contact us or submit your feedback about this product.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Copyright © 2024 Creative Biolabs. All Rights Reserved.