We employ optimized culturing techniques to isolate E. faecalis from complex samples (such as fecal matter or environmental swabs) and obtain pure colonies. Our team then performs initial screening of isolates, including Gram staining and genotypic confirmation, to verify species identity. We also evaluate colony morphology and basic phenotypic traits to select robust E. faecalis strains for downstream analyses, providing a solid foundation of high-quality isolates for your research.
Creative Biolabs offers comprehensive antibiotic susceptibility profiling for E. faecalis strains. Using standardized assays (e.g. broth microdilution and disk diffusion), we determine the minimum inhibitory concentrations of various antibiotics and chart the resistance/susceptibility profile of each strain. This is especially important given the rise of vancomycin-resistant E. faecalis isolates in recent years. Our detailed reports identify any antibiotic resistance genes or phenotypes present, helping researchers understand safety concerns and informing the development of new antimicrobial strategies.
We conduct extensive functional assays to uncover the biochemical activities and potential mechanisms of action (MoA) of E. faecalis strains. These screenings include tests for metabolic capabilitiesand beneficial functions like bile salt tolerance, short-chain fatty acid production, or bacteriocin secretion. We also examine antagonistic activity against pathogens and other microbiota members. By elucidating E. faecalis' functional attributes, our service helps pinpoint how a strain exerts its effects within the microbiome and provides clues to its probiotic potential or pathogenic mechanisms.
Understanding how E. faecalis interacts with host tissues is key to gauging its safety and efficacy. We offer in vitro host-microbe interaction assays, including adhesion studies with intestinal epithelial cell lines and gut barrier integrity tests. For example, we can quantify E. faecalis' ability to adhere to mucosal surfaces or its effect on tightening or loosening of epithelial junctions. We also assess cross-talk with host signaling pathways by measuring changes in epithelial cell responses upon co-culture.
Our scientists evaluate the immunomodulatory properties of E. faecalis using specialized cell-based assays. We incubate live E. faecalis with immune cells such as human peripheral blood mononuclear cells or macrophage/DC cultures. By monitoring cytokine production (e.g. levels of IL-10, IL-12, TNF-α) and immune cell activation markers, we determine whether a given E. faecalis strain induces pro-inflammatory or anti-inflammatory responses. These in vitro immune modulation tests are crucial for identifying strains that could be beneficial versus those that trigger undesirable immune reactions.
Creative Biolabs provides fermentation process development tailored to E. faecalis. Thanks to their tolerance to a range of pH and temperature condition, E. faecalis strains can be cultured at scale efficiently. We optimize growth media and bioreactor parameters to maximize biomass and maintain strain viability and functionality. Whether you require small pilot batches or larger quantities, our fermentation experts ensure a consistent, high-yield production of E. faecalis. This service is invaluable for generating sufficient material for animal studies, formulation trials, or further product development.
After culturing the bacteria, we also support the formulation of E. faecalis as a stable product for research use. Our formulation services include concentration and purification of the cultured bacteria, followed by processes such as lyophilization (freeze-drying) to create a shelf-stable powder. We carefully select cryoprotectants and excipients that preserve cell viability and activity during storage. Additionally, we can formulate E. faecalis into capsules, suspensions, or other delivery formats as needed for your experimental design.
We offer a battery of safety evaluations for E. faecalis strains to ensure they are suitable for research. Our safety tests include checking for known virulence factors and toxin production, assessing hemolytic activity on blood agar, and measuring undesirable metabolites like biogenic amines. We also confirm the absence of transferable antibiotic resistance genes to avoid propagating dangerous traits. For example, a strain intended as a probiotic candidate should show no hemolysis, no toxin gene sequences, and be susceptible to standard antibiotics. By thoroughly screening each strain, Creative Biolabs helps you meet regulatory guidelines and ensures E. faecalis is handled with appropriate biosafety precautions.
