We isolate and purify strictly anaerobic A. rhamnosivorans from stools, biobanks, or consortia using oxygen-free cultivation, reducing media, and selective pressures. High-throughput screening ranks clones by substrate use and butyrate yield under controlled pH and redox. Outputs de-risk downstream MoA assays and scale-up, with strain passports for A. rhamnosivorans continuity.
We confirm A. rhamnosivorans via 16S rRNA and whole-genome sequencing, phylogenomics, ANI, and MLST when needed. Our reports differentiate closely related taxa and intraspecies variants, documenting genomic determinants for lactate-to-butyrate conversion. This ensures your A. rhamnosivorans datasets remain traceable, reproducible, and comparable across experiments, sites, and time.
We quantify how A. rhamnosivorans metabolizes rhamnose, glucose, lactate/acetate co-substrates, and other carbohydrates across pH and redox windows. GC/LC readouts map butyrate/propionate distributions and carbon recovery. Data drive media composition and feeding strategies tailored to A. rhamnosivorans, informing formulation design and co-culture compatibility with lactate donors.
We design functional screens around A. rhamnosivorans “lactate clearance → butyrate generation” pathways, barrier-support proxies, and cross-feeding logic. Multi-omics (metabolomics, transcriptomics) and isotope tracing connect A. rhamnosivorans flux with defined endpoints. Decision matrices identify strains and conditions that maximize reproducible butyrate output.
Using epithelial monolayers, organoids, and co-culture models, we track A. rhamnosivorans adhesion patterns, mucin interactions, TEER, tight-junction markers, and SCFA signaling. Readouts link host responses to A. rhamnosivorans phenotypes, revealing how media, substrates, and oxygen exposure shape barrier-relevant outcomes in a standardized, RUO-aligned system.
We expose primary cells or lines to cell-free supernatants and defined fractions from A. rhamnosivorans. Cytokines, chemokines, and pathway nodes (e.g., NF-κB, MAPK) are quantified to compare strains and culture regimes. The platform clarifies how A. rhamnosivorans inputs modulate immune readouts without implying clinical claims.
We optimize strict-anaerobe bioprocess parameters for A. rhamnosivorans: inoculum density, pH control, reducing potential, carbon feeding, and fed-batch/chemostat modes. Online/at-line analytics follow butyrate trajectories and biomass. Results produce reproducible DoE-derived windows for A. rhamnosivorans scale-up, tech transfer, and downstream formulation.
We develop stabilization strategies for A. rhamnosivorans, including protectant blends, oxygen-mitigating excipients, and drying/packaging conditions. Stress-challenge and shelf-life studies quantify CFU retention and functional potency. The deliverable is a stability playbook that preserves A. rhamnosivorans viability during storage, shipment, and deployment.
