Anaerostipes rhamnosivorans as Next Generation Probiotics

Butyrate is a four-carbon SCFA, mainly known as a fuel for colonocytes. Butyrate has a wide range of pharmacological activities, including microbiome modulators, anti-obesity, anti-inflammation, anti-angiogenesis, metabolic pathway modulators, and antioxidant. Therefore, some bacteria that produce butyrate are potentially promising therapeutic targets for diseases. At Creative Biolabs, we are highly experienced in the development of live biotherapeutic products (LBP), including many candidate strains with potential therapeutic effects.

Introduction of Anaerostipes rhamnosivorans (A. rhamnosivorans)

A. rhamnosivorans is a butyrate-producing bacterium, newly isolated from infant stool. This strain belongs to the genus Anaerostipes, which is among the top fifteen abundant genera in the human intestinal microbiome. A. rhamnosivorans cells are non-motile, spore-forming, anaerobic rods and are often observed to elongate and form curly cells in old cultures. Cells are 0.70-0.83×3-6μm. They occur as single cells, sometimes in short chains of two to five cells. They stain Gram-positive (young cells) or Gram-negative (old cells). On RCM agar, colonies are beige, circular, and raised with rough surfaces, about 1-4 mm in diameter. Can grow at 15-45℃ (optimum at 37℃), pH 5.5-8.0 (optimum pH 6.5-7.0) and up to 3% NaCl. Lactate, formate, butyrate, and acetate are major metabolites from glucose fermentation, and propane-1, 2-diol, butyrate, acetate, and formate are major products of rhamnose fermentation. The type strain is 1y-2T (DSM 26241T=5KCTC 15316T), isolated from a stool of a healthy infant in Wageningen, Netherlands, in 2012. The DNA G+C content of the type strain is 44.5 mol%.

All butyrate-producing bacteria in different clostridial clusters. Fig.1 All butyrate-producing bacteria in different clostridial clusters. (Amiri, 2021)

The Potential Benefits of A. rhamnosivorans

Mice fed with live myo-inositol-fermenting A. rhamnosivorans had reduced fasting glucose levels as compared to mice fed with heat-killed A. rhamnosivorans and the reduction of fasting glucose level became significant once A. rhamnosivorans was supplemented with myo-inositol. This suggests that the observed benefits of A. rhamnosivorans may be related to its active fermentation of administered myo-inositol, resulting in increased production of beneficial compounds. It would be interesting to study the effect of Anaerostipes spp. on improving glucose tolerance in patients with metabolic syndrome or type 2 diabetes.

  • Colonic Health

Butyricogenic bacteria and their role in the colonic anaerobic food chain are of great concern because butyrate is beneficial to intestinal health. Scientists set up experimental in vitro co-culture systems to study the metabolic interactions of Anaerostipes rhamnosivorans, a butyrate producer with each of its partners. In all co-cultures, A. rhamnosivorans benefited from its partner, promoting butyrate formation, compared with monoculture. The findings indicate enhanced butyrate formation through microbe-microbe interactions between A. rhamnosivorans and an acetogen, a methanogen, or a pectin degrader. This microbial interaction that promotes butyrate formation may be beneficial for colon health.

2 Schematic illustration of the interactions between<em>A. rhamnosivorans</em> and intestinal microbes in different substrates. Fig.2 Schematic illustration of the interactions betweenA. rhamnosivorans and intestinal microbes in different substrates. (Bui, 2019)

What Services Can We Provide for A. rhamnosivorans at Creative Biolabs?

A. rhamnosivorans Related Products at Creative Biolabs

  • Strain Products

We supply a variety of A. rhamnosivorans strains of different preservation numbers as below. If you need other strains, please contact us.

  • Customized strain culture supernatant. (e.g.: for animal research)
  • Customized strain lyophilized powder containing certain CFU. (e.g.: for animal research)

High butyrate production can be used as a trait to design microbe mixtures for therapeutic purposes. Creative Biolabs’ scientists and engineers have the experience and expertise to help solve challenges. We will work with you to develop and deliver testing and analytical solutions for LBP projects. Please feel free to contact us, we are happy for further discussion.

References

  1. Amiri, P.; et al. Role of Butyrate, a Gut Microbiota Derived Metabolite, in Cardiovascular Diseases: A comprehensive narrative review. Frontiers in Pharmacology. 2021, 12.
  2. Bui, T.P.N.; et al. Mutual metabolic interactions in co-cultures of the intestinal Anaerostipes rhamnosivorans with an acetogen, methanogen, or pectin-degrader affecting butyrate production. Frontiers in microbiology. 2019: 2449.

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