Bifidobacterium breve is a Gram-positive, anaerobic, rod shaped organism that is non motile and forms branches with its neighbors.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-055FG |
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Product Information | |
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Product Overview | Bifidobacterium breve is a Gram-positive, anaerobic, rod shaped organism that is non motile and forms branches with its neighbors. |
Target | Bifidobacterium |
Genus | Bifidobacterium |
Application | Study and research |
Type Strain | No |
Culture Medium | BBL |
Culture Conditions | 37°C; Anaerobic |
Characteristics | Gram-positive, bacillus, arginine double hydrolase negative, urease positive. It can ferment glucose, raffinose, does not ferment trehalose, arabinose, xylose. The optimum growth temperature is 37- 41°C. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Bifidobacterium are Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They are originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS; Generally Recognised As Safe status. |
Alternative Names | Bifidobacterium breve |
To revive Bifidobacterium breve 15700, first, the lyophilized culture should be rehydrated using a suitable anaerobic medium. Dissolve the contents in the recommended rehydration fluid, such as the Culti-Loops Aerobic Set Rehydration Fluid. Then, streak the culture onto an appropriate anaerobic agar medium and incubate at 37°C under anaerobic conditions to ensure optimal growth.
For long-term storage, Bifidobacterium breve should be stored at -80°C. The culture should be aliquoted into cryovials with a suitable cryoprotectant, such as 15% glycerol, to prevent ice crystal formation and maintain cell viability during freezing and thawing processes.
Bifidobacterium breve is widely used in studies focused on gut microbiota, probiotic functionalities, and metabolic interactions between gut bacteria and dietary components. It is particularly valuable in research exploring the beneficial effects of probiotics on human health, including the modulation of the immune system and the enhancement of intestinal barrier function.
Ensure the genetic integrity and viability of Bifidobacterium breve through whole-genome sequencing and regular viability assays. Adhere to the recommended storage and handling procedures to maintain the culture's viability. Use only validated media and reagents as specified in the product documentation to ensure consistent and reliable experimental outcomes.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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