Bifidobacterium infantis is a subspecies of the Bifidobacterium longum species. It is an inherited microbe, often being passed down from mother to child during child birth.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-060FG |
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Product Information | |
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Product Overview | Bifidobacterium infantis is a subspecies of the Bifidobacterium longum species. It is an inherited microbe, often being passed down from mother to child during child birth. |
Target | Bifidobacterium |
Genus | Bifidobacterium |
Application | Study and research |
Type Strain | Yes |
Culture Medium | BBL |
Culture Conditions | 37°C; Anaerobic |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Bifidobacterium are Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They wre originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS; Generally Recognised As Safe status. |
Alternative Names | Bifidobacterium infantis Reuter |
Bifidobacterium infantis should be handled using an anaerobic chamber or jars with anaerobic gas packs. Ensure all manipulations are done quickly to minimize exposure to oxygen. Using loose screw caps and sterile butyl rubber stoppers can help retain anaerobic conditions.
For genomic studies, Bifidobacterium infantis should be cultured to mid-log phase in an anaerobic environment. Harvest cells by centrifugation and extract DNA using a high-quality genomic DNA isolation kit. Ensuring high-purity DNA is crucial for downstream applications such as sequencing.
Rehydrating lyophilized Bifidobacterium infantis involves adding the lyophilized pellet to a pre-warmed anaerobic medium, followed by gentle mixing. Incubate the rehydrated culture in an anaerobic environment at 37°C until the culture reaches the desired density, ensuring minimal oxygen exposure during the process.
Store Bifidobacterium infantis at -80°C in a cryoprotectant such as glycerol or skim milk. Avoid repeated freeze-thaw cycles to prevent loss of viability. Aliquoting the culture into small volumes can help minimize thawing events.
To assess the activity of Bifidobacterium infantis after storage, revive the culture in an anaerobic medium and monitor growth parameters such as optical density and colony-forming units. Additionally, functional assays such as short-chain fatty acid production can be performed to confirm activity.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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