Bifidobacterium animalis subsp. lactis 420, known for its immunoregulatory properties and improving intestinal epithelial integrity in mice models, has been given to humans in earlier clinical trials.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBGF-0722-GF64
Product Information
Product Overview
Bifidobacterium animalis subsp. lactis 420, known for its immunoregulatory properties and improving intestinal epithelial integrity in mice models, has been given to humans in earlier clinical trials.
Target
Bifidobacterium
Genus
Bifidobacterium
Application
Study and research
Culture Medium
BBL
Culture Conditions
37°C; Anaerobic
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bifidobacterium is Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They wre originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS.
Alternative Names
Bifidobacterium animalis subsp. lactis 420
FAQsPublished Data Customer Reviews Related Products
How should Bifidobacterium animalis subsp. lactis be stored before use in experiments?
Bifidobacterium animalis subsp. lactis should be stored at -80°C to maintain its viability. Prior to use, it should be thawed on ice to preserve its integrity. This ensures the bacteria remain active for accurate experimental results.
What is the recommended medium for culturing Bifidobacterium animalis subsp. lactis ?
Bifidobacterium animalis subsp. lactis is typically cultured in MRS broth or agar under anaerobic conditions. This medium supports optimal growth and viability, essential for reliable experimental outcomes.
What are the optimal growth conditions for Bifidobacterium animalis subsp. lactis ?
The optimal growth conditions for Bifidobacterium animalis subsp. lactis include an anaerobic environment, a temperature of 37°C, and a pH of around 6.5-7.0. These conditions promote robust growth and activity.
How should the concentration of Bifidobacterium animalis subsp. lactis be determined before an experiment?
The concentration of Bifidobacterium animalis subsp. lactis can be determined using optical density measurements at 600 nm (OD600) or colony-forming unit (CFU) counts on MRS agar plates. Accurate concentration determination is crucial for experimental reproducibility.
What methods can be used to assess the viability of Bifidobacterium animalis subsp. lactis in experiments?
Viability of Bifidobacterium animalis subsp. lactis can be assessed using live/dead staining techniques, CFU counts, or flow cytometry. These methods help in determining the proportion of live bacteria, ensuring the accuracy of experimental data.
B420's impact on metabolic health
The study reviewed the impact of Bifidobacterium animalis subsp. lactis 420 (B420) on metabolic health, particularly its role in weight management and glycemic control. The research encompassed in vitro, pre-clinical, and clinical studies. In a human intervention trial, B420 significantly reduced total body fat mass and waist circumference in overweight and obese adults. For example, there was a 4% reduction in total body fat and a 2.4% reduction in waist circumference in the B420 group compared to the placebo group. Pre-clinical studies demonstrated that B420 enhanced mucosal integrity and reduced inflammation by lowering the expression of pro-inflammatory cytokines like IL-6 and TNF-α in diabetic and obese mouse models. Additionally, B420 improved glycemic control by reducing fasting blood glucose levels and enhancing insulin sensitivity. These findings suggest that B420 can modulate gut microbiota composition, improving gut barrier function and metabolic health. By increasing the prevalence of beneficial microbes and reducing systemic inflammation, B420 shows potential as a therapeutic probiotic for managing obesity and associated metabolic disorders.
Uusitupa, H. M., Rasinkangas, P., et al. Bifidobacterium animalis subsp. lactis 420 for Metabolic Health: Review of the Research. Nutrients. 2020, 12(4): 892. Distributed under Open Access license CC BY 4.0, without modification.
Exceptional Viability Bifidobacterium animalis subsp. lactis 420 from Creative Biolabs showed exceptional viability, significantly enhancing gut microbiome research.
Consistent Quality
The consistent quality of Bifidobacterium animalis subsp. lactis 420 ensures reliable results in metabolic studies. Creative Biolabs delivers a product that outperforms competitors in every aspect.
Outstanding Performance in Inflammation Research Bifidobacterium animalis subsp. lactis 420 demonstrated outstanding performance in inflammation research, offering reproducible results. Creative Biolabs' strain is a superior choice for high-impact studies.
Reliable and Effective
This strain is reliable and effective in gastrointestinal research. Bifidobacterium animalis subsp. lactis 420 from Creative Biolabs provides consistent results, outperforming other available options.
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