Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species. |
Target | Lactobacillus |
Genus | Lactobacillus |
Application | Study and research |
Type Strain | No |
Culture Medium | MRS |
Culture Conditions | 37°C; Anaerobic |
Characteristics | Gram-positive bacteria, rod-shaped, facultative anaerobic, without spores and movement, no hydrogen sulfide production. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel. |
Alternative Names | Lactobacillus crispatus; 243 |
Lactobacillus crispatus; 243 grows best in anaerobic or microaerophilic conditions, at 37°C in MRS broth with a pH of 5.5-6.0, favoring slightly acidic environments.
To maintain the viability of Lactobacillus crispatus; 243, it is essential to store it at -80°C if frozen, or below 4°C for short-term use in a liquid culture medium. Avoid repeated freeze-thaw cycles, as this can lead to significant loss in bacterial viability. Proper storage ensures the strain remains potent for downstream research applications.
Lactobacillus crispatus; 243 is used in research on vaginal health, bacterial vaginosis prevention, and microbiome studies, with applications in probiotic therapy and pathogen defense.
Viability is typically assessed through colony-forming units (CFU) counting, where the bacterial culture is serially diluted and plated on selective agar to estimate live bacterial counts. Alternative methods such as flow cytometry or qPCR targeting Lactobacillus crispatus-specific genes can also be used to determine the bacterial load in complex microbial communities.
Scaling up Lactobacillus crispatus; 243 from lab-scale to industrial production involves optimizing fermentation conditions, ensuring high cell viability, and maintaining consistent product quality. Common challenges include maintaining bacterial stability during production, storage, and delivery in commercial probiotic formulations. Addressing these issues is critical for effective live biotherapeutic product development.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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