Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species that was isolated from the vagina of a healthy person.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-117FG |
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Product Information | |
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Product Overview | Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species that was isolated from the vagina of a healthy person. |
Target | Lactobacillus |
Genus | Lactobacillus |
Application | Study and research |
Type Strain | No |
Culture Medium | MRS |
Culture Conditions | 37°C; Anaerobic |
Source | The vagina of a healthy person |
Characteristics | The thalli are rod-shaped, singly or in pairs, gram-positive. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel. |
Alternative Names | Lactobacillus crispatus; 24879 |
Lactobacillus crispatus; 24879 typically thrives under anaerobic or microaerophilic conditions at a temperature of around 37°C. It is commonly cultured in MRS broth, which provides the nutrients necessary for growth. pH monitoring and controlling the anaerobic environment are essential for successful culture.
Lactobacillus crispatus is often used in gut microbiota studies due to its ability to adhere to epithelial cells and modulate the immune response. In studies, the strain can be administered orally to animal models or cultured in vitro to observe interactions with other microbiota species. The strain's probiotic effects are significant for studying vaginal and gut health.
In co-culture experiments, Lactobacillus crispatus; 24879 can be combined with other bacterial species to study microbial interactions. Optimizing growth media and adjusting culture conditions for each bacterial species is essential. Researchers should monitor the growth kinetics to avoid overgrowth of one species and ensure balanced co-culture dynamics.
Culturing Lactobacillus crispatus; 24879 in anaerobic conditions can present challenges such as contamination risks and maintaining a stable anaerobic environment. Researchers must closely monitor the culture conditions, including pH and temperature, to ensure optimal growth. Regular media changes and aseptic techniques are required to maintain culture integrity.
In biofilm formation studies, Lactobacillus crispatus; 24879 can be seeded on surfaces such as glass or plastic and incubated under anaerobic conditions. Biofilm formation is typically monitored using microscopy or crystal violet staining assays. This allows researchers to study the strain's ability to adhere to surfaces and form protective layers, which is relevant for probiotic and microbiota research.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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