Anaerobutyricum hallii was isolated from human faeces.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Anaerobutyricum hallii was isolated from human faeces.
Target
Anaerobutyricum
Genus
Anaerobutyricum
Strain Designation
VPI B4-27
Application
Study and research
Type Strain
Yes
Culture Medium
Medium 110
Culture Conditions
37°C; Anaerobic
Source
Human faeces
Risk Group
1
Genbank Accession Numbers
Whole genome shotgun sequence: ACEP00000000
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Anaerobutyricum is an anaerobic bacterial genus that lives inside the human digestive system.
Alternative Names
Anaerobutyricum hallii
FAQsPublished Data Customer Reviews Related Products
How should Anaerobutyricum hallii be stored before use in experiments?
Anaerobutyricum hallii should be stored in an anaerobic environment at -80°C to preserve its viability and functionality. It is critical to avoid repeated freeze-thaw cycles, which can significantly diminish the bacterial activity and integrity.
What is the recommended inoculum size for Anaerobutyricum hallii in laboratory cultures?
For optimal experimental outcomes, an inoculum size of approximately 1 × 10^6 CFU/mL is recommended. This concentration ensures robust growth and metabolic activity, suitable for various research applications.
Which medium is suitable for culturing Anaerobutyricum hallii?
Anaerobutyricum hallii is best cultured in YCFA (Yeast extract, Casitone, Fatty Acid) medium, supplemented with glucose, lactate, and glycerol. This medium supports its growth and metabolic processes, including the production of short-chain fatty acids such as butyrate and propionate.
How long does it take for Anaerobutyricum hallii to reach log phase growth?
Under optimal anaerobic conditions at 37°C, Anaerobutyricum hallii typically reaches log phase growth within 12-18 hours. Monitoring the growth curve is essential for determining the precise time to harvest the cells for downstream applications.
What are the steps involved in preparing Anaerobutyricum hallii for in vivo studies?
Preparation for in vivo studies involves growing the culture to the desired phase, harvesting the cells via centrifugation, and resuspending them in a suitable carrier, such as glycerol or phosphate-buffered saline (PBS). This preparation ensures accurate dosing and consistency in experimental applications.
Impact of supplementing microbiomes with A. hallii on PhIP transformation, glycerol metabolism, and SCFAs in batch-fermented fecal microbiota
The research focused on the role of Anaerobutyricum hallii in the transformation of the dietary carcinogen PhIP in diverse human fecal microbiota. Utilizing shotgun metagenomics, the study analyzed the taxonomic profiles and the abundance of glycerol/diol dehydratase (gdh)-harboring taxa in fecal samples from 20 healthy adults. The results revealed that A. hallii was the dominant gdh-harboring taxon in 18 of the 20 samples, contributing significantly to PhIP transformation. Fecal microbiota with a high abundance of A. hallii transformed more PhIP than those with low abundance. Supplementing microbiota with A. hallii further increased PhIP transformation capacity, demonstrating its potential in reducing carcinogen exposure. Experimentally, supplementing fecal microbiota with A. hallii increased PhIP transformation from 0% to 4% after 6 hours in microbiota with initially low A. hallii levels. This enhancement in PhIP transformation capacity was associated with an increased production of acrolein, a reactive metabolite. The findings suggest that A. hallii supplementation could be a viable strategy for mitigating the mutagenic effects of dietary carcinogens, emphasizing its significance in promoting gut health and reducing cancer risk.
Ramirez Garcia, A., Greppi, A., et al. Anaerobutyricum hallii promotes the functional depletion of a food carcinogen in diverse healthy fecal microbiota. Frontiers in Microbiomes. 2023, 2: 1194516. Distributed under Open Access license CC BY 4.0, without modification.
Seamless Integration into Protocols
The Anaerobutyricum hallii from Creative Biolabs integrates seamlessly into research protocols, enhancing workflow efficiency and consistency.
Reliable Culture Performance
This product delivers reliable performance in bacterial cultures, ensuring robust growth and consistent activity in all experiments.
User-Friendly Application
Creative Biolabs' Anaerobutyricum hallii is user-friendly, facilitating smooth application in laboratory processes and improving research productivity.
Reliable Viability Post-Storage
Even after prolonged storage, this product maintains high viability, ensuring that experimental results are not compromised.
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