Bifidobacterium longum is a Gram-positive, catalase-negative, rod-shaped bacterium present in the human gastrointestinal tract.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-057FG
Product Information
Product Overview
Bifidobacterium longum is a Gram-positive, catalase-negative, rod-shaped bacterium present in the human gastrointestinal tract.
Target
Bifidobacterium
Genus
Bifidobacterium
Application
Study and research
Type Strain
Yes
Culture Medium
CM0233
Culture Conditions
37°C; Anaerobic
Characteristics
The formation of biofilm is conducive to adhesion and colonization.
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bifidobacterium are Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They wre originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS; Generally Recognised As Safe status.
Alternative Names
Bifidobacterium longum subsp. infantis
FAQsPublished Data Customer Reviews Related Products
How is Bifidobacterium longum subsp. infantis prepared for research applications?
Bifidobacterium longum subsp. infantis is typically prepared by fermentation, followed by freezing and freeze-drying to maintain viability. The bacteria are often mixed with cryoprotectants during preparation. For use, the freeze-dried powder is reconstituted in an appropriate medium or directly added to the experimental setup. Proper preparation is essential to ensure the stability and efficacy of the bacteria throughout the research process.
What controls are necessary when using Bifidobacterium longum subsp. infantis in laboratory experiments?
When using Bifidobacterium longum subsp. infantis in experiments, it is critical to include appropriate controls such as placebo groups or non-treated samples. This helps in accurately determining the effects of the bacteria. Additionally, ensuring that the bacterial preparation is free from contaminants and adheres to strain-specific standards is crucial for the validity of the study.
What are the recommended protocols for culturing Bifidobacterium longum subsp. infantis in the lab?
For culturing Bifidobacterium longum subsp. infantis, a suitable anaerobic environment must be maintained. The bacteria should be cultured in a medium rich in nutrients that support its growth, such as MRS broth. Incubation typically occurs at 37°C for 24-48 hours. Maintaining anaerobic conditions is critical to ensure the proper growth and activity of the bacteria.
How can the stability of Bifidobacterium longum subsp. infantis be ensured during long-term storage?
The stability of Bifidobacterium longum subsp. infantis during long-term storage can be ensured by keeping it at -80°C in a lyophilized form. Adding cryoprotectants during the preparation process can protect the bacteria from damage during freezing and thawing. Regular viability checks are recommended to ensure the bacteria remain effective for research purposes.
What are the common quality control measures for verifying the identity and purity of Bifidobacterium longum subsp. infantis?
Common quality control measures for Bifidobacterium longum subsp. infantis include DNA sequencing, PCR-based methods, and phenotypic analysis to verify the identity of the strain. Purity can be assessed by culturing the bacteria on selective media and conducting contaminant testing. Ensuring the strain's genetic stability and purity is crucial for obtaining accurate and reliable research results.
Growth on HMOs of select B. infantis strains
The study highlights the genetic variability in the utilization of human milk oligosaccharides (HMOs) among B. infantis strains. The study analyzed twelve probiotic strains and identified two key variants in the HMO utilization gene clusters. Specifically, some strains, termed H5-positive, possessed a full set of HMO utilization genes, including an ABC-type transporter, while H5-negative strains lacked this transporter. This genetic distinction resulted in significant differences in the strains' growth capabilities on specific HMOs, such as lacto-N-tetraose and lacto-N-neotetraose. The experimental data underscored that H5-positive strains exhibited superior growth in both in vitro and in vivo conditions. For instance, the study demonstrated that in breastfed infants, H5-positive strains showed a significant fitness advantage over H5-negative strains. This research underscores the importance of genetic profiling in selecting probiotic strains for infant gut health, highlighting that the presence of specific HMO utilization genes can greatly enhance the efficacy of B. infantis strains in promoting a healthy infant microbiome.
Duar, Rebbeca M., et al. "Comparative genome analysis of Bifidobacterium longum subsp. infantis strains reveals variation in human milk oligosaccharide utilization genes among commercial probiotics." Nutrients 12.11 (2020): 3247. Distributed under Open Access license CC BY 4.0, without modification.
Superior Efficacy in Gut Health Research Bifidobacterium longum subsp. infantis from Creative Biolabs demonstrated exceptional efficacy in modulating gut microbiota and enhancing intestinal barrier function in experimental models.
Outstanding Quality and Reliability
The Bifidobacterium longum subsp. infantis provided by Creative Biolabs is of outstanding quality, ensuring consistent and reliable results in immunological studies.
Enhanced Metabolic Research Outcomes
This strain has proven invaluable in metabolic research, particularly in studying the effects of probiotics on lipid metabolism. Its consistency and reliability stand out in comparative studies.
Excellent Performance in Inflammatory Response Studies
Creative Biolabs' Bifidobacterium longum subsp. infantis excels in studies on inflammation, significantly reducing pro-inflammatory markers. Ideal for researchers focusing on immune modulation.
Reliable Results in Microbiota Composition Analysis
The product showed remarkable stability and effectiveness in altering gut microbiota composition, outperforming other brands in comparative analysis.
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