Organoid Technology for Live Biotherapeutic Products Assessment
Creative Biolabs specialized in live biotherapeutics and next-generation probiotics research. For more than a decade, we are dedicated to providing professional, tailored customer service, helping numerous customers with outstanding customer service, and winning a global reputation.
Introduction of Organoids Technology
Probiotics, or beneficial, bacteria, hold promise for the treatment of human disease. More study is needed to fully realize the potential of probiotics. Safety and efficacy studies are critically important. 3D culture systems make it possible to partially reproduce the complexity of mammalian organogenesis in vitro. Newly developed organoid systems act as a bridge between in vitro and in vivo systems. Organoids are one of the most accessible and physiologically relevant models to study the dynamics of host-microbial interaction in a controlled environment.
Organoids Technology at Creative Biolabs
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Study Host-microbial Interactions
The development of intestinal organoid cultures is a major technological advance in the field of stem cells. Organoids provide a novel and powerful ex vivo model for studying commensal bacteria, probiotics, and microbiome studies. A recent study examined the transcriptional response of organoids exposed to short-chain fatty acids and products produced by two abundant microbiota components (Akkermansia muciniphila and Faecalibacterium prausnitzii). Metabolites of Akkermansia muciniphila affect a variety of transcription factors and genes involved in cellular lipid metabolism and growth, supporting previous in vivo findings.
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Personalized Disease Models
Parallel co-cultures of IBD-derived and matched healthy organoids followed by the addition of patient-derived microbiota and immune cells could also clarify whether loss of tolerance to microbiota is a cause or consequence of disease, a fundamental question in IBD research.
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Omics and Single-cell Approaches
Recently, single-cell omics approaches have been applied to characterize individual cells in murine organoids. Similar approaches could provide new insights into the mechanisms by which gut microbiota or specific probiotics influence the function of specific subsets of cells associated with IBD.
Patient-derived organoids can be used to study drug resistance, metabolism, differentiation, and cancer gene function. Drug screening, however, is not limited to cancer therapies, and organoids have also been used successfully to screen drugs for a variety of other diseases, including cystic fibrosis and Zika virus infection, further indicating the usefulness of these models.
Advantages of Organoids System
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Near-physiological models of epithelial tissue contain heterogeneous cell populations that maintain structural integrity in vivo.
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Capable of analyzing cell-cell and cell-matrix interactions while maintaining mechanical properties, gene expression, and metabolic profiles.
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Ex vivo culture bridges the gap between cell culture and animal modeling.
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Can microinject microbes into the lumen of organoids to study host-pathogen interactions.
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Organoid/Enteroids can be grown in two-dimensional monolayers of Transwell inserts so that microorganisms can be exposed to apical cell surfaces simultaneously with basolateral immune cells, which are more representative of the human microenvironment in vivo.
Fig.1 The intestinal epithelium and the organoid model. (Poletti, 2021)
Creative Biolabs is proud to support clients with professional scientists, experienced researchers, advanced instruments, proprietary technology platforms, and optimized processes. As a global leader in live biotherapeutics, we have the necessary knowledge to deliver the best results. If you are interested in our organoid technology, please feel free to contact us for more.
Reference
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Poletti, M.; et al. Organoid-based models to study the role of host-microbiota interactions in IBD. Journal of Crohn's and Colitis. 2021, 15 (7): 1222-1235.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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